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Literature summary for 1.14.16.4 extracted from
- Properties of tryptophan hydroxylase from human carcinoid tumor (1977), Biochim. Biophys. Acta, 482, 27-34.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 0.0024 mM, 50% inhibition, competitive vs. tryptophan | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 1.2% O2 with 0.2 mM 2-amino-4-hydroxy-6-methyl-5,6,7,8-tetrahydropteridine, 7.1% O2 with 0.4 mM 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine | Homo sapiens | |
| 0.05 | - |
2-amino-4-hydroxy-6-methyl-5,6,7,8-tetrahydropteridine | - |
Homo sapiens | |
| 0.105 | - |
2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | not required for full activity | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
enzyme from a carcinoid tumor | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| carcinoma cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000018 | - |
enzyme from carconoid tumor | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, under N2, 2 months, no loss of activity | Homo sapiens |
| 4°C, under O2, 48 h, 90% loss of activity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tryptophan + 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine + O2 | - |
Homo sapiens | ? | - |
r | |
| L-tryptophan + tetrahydropteridine + O2 | - |
Homo sapiens | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
in the presence of 0.2 mM Fe2+ and 2-mercaptoethanol | Homo sapiens |
| 6.8 | - |
in the presence of 0.2 mM Fe2+, without 2-mercaptoethanol | Homo sapiens |
| 7.2 | - |
in the presence of 35.5 mM 2-mercaptoethanol | Homo sapiens |
| 7.6 | - |
without 2-mercaptoethanol and Fe2+ | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00082 | - |
1,10-phenanthroline | vs. tryptophan | Homo sapiens | |
| 0.0026 | - |
1,10-phenanthroline | vs. 2-amino-4-hydroxy-6-methyl-5,6,7,8-tetrahydropteridine | Homo sapiens | |
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