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Literature summary for 1.14.16.2 extracted from
- Catalytic domain surface residues mediating catecholamine inhibition in tyrosine hydroxylase (2014), J. Biochem., 155, 183-193 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A297L | the mutation mediates high affinity dopamine inhibition through Vmax reduction and increasing the Km value for the cofactor | Rattus norvegicus |
| D299N | inactive | Rattus norvegicus |
| D361N | the mutant shows increased Vmax compared to the wild type enzyme | Rattus norvegicus |
| E362Q | the mutant shows reduced Vmax compared to the wild type enzyme | Rattus norvegicus |
| E362R/E365R | the mutation mediates high affinity dopamine inhibition through Vmax reduction and increasing the Km value for the cofactor | Rattus norvegicus |
| E365Q | the mutant shows reduced Vmax compared to the wild type enzyme | Rattus norvegicus |
| K170E/L480A | inactive | Rattus norvegicus |
| K366L | the mutant shows reduced Vmax compared to the wild type enzyme | Rattus norvegicus |
| R298L | inactive | Rattus norvegicus |
| R37E/R38E | the Km value for tetrahydrobiopterin measured for the mutant is approximately half that of the wild type enzyme | Rattus norvegicus |
| S368A | the mutation mediates high affinity dopamine inhibition through Vmax reduction and increasing the Km value for the cofactor | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dopamine | 1.8% residual activity at 0.01 mM | Rattus norvegicus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 300000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tyrosine + tetrahydrobiopterin + O2 | Rattus norvegicus | - |
L-dopa + 4a-hydroxytetrahydrobiopterin + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | P04177 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | - |
Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Ni-NTA column chromatography, and Superdex 200 gel filtration | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tyrosine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | L-dopa + 4a-hydroxytetrahydrobiopterin + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 70000, calculated from amino acid sequence | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tyrosine hydroxylase | - |
Rattus norvegicus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.000595 | - |
pH and temperature not specified in the publication | Rattus norvegicus | dopamine | |
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Release 2023.1 (January 2023)
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