Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D425A | the mutant shows strongly reduced activity compared to the wild type enzyme | Rattus norvegicus |
D425C | the mutant shows strongly reduced activity compared to the wild type enzyme | Rattus norvegicus |
D425E | the mutant shows strongly reduced activity compared to the wild type enzyme | Rattus norvegicus |
D425F | the mutant shows strongly reduced activity compared to the wild type enzyme | Rattus norvegicus |
D425G | the mutant shows strongly reduced activity compared to the wild type enzyme | Rattus norvegicus |
D425H | the mutant shows strongly reduced activity compared to the wild type enzyme | Rattus norvegicus |
D425I | the mutant shows strongly reduced activity compared to the wild type enzyme | Rattus norvegicus |
D425K | the mutant shows strongly reduced activity compared to the wild type enzyme | Rattus norvegicus |
D425L | the mutant shows strongly reduced activity compared to the wild type enzyme | Rattus norvegicus |
D425M | the mutant shows strongly reduced activity compared to the wild type enzyme | Rattus norvegicus |
D425N | the mutant shows strongly reduced activity compared to the wild type enzyme | Rattus norvegicus |
D425Q | the mutant shows strongly reduced activity compared to the wild type enzyme | Rattus norvegicus |
D425R | the mutant shows strongly reduced activity compared to the wild type enzyme | Rattus norvegicus |
D425S | the mutant shows strongly reduced activity compared to the wild type enzyme | Rattus norvegicus |
D425T | the mutant shows strongly reduced activity compared to the wild type enzyme | Rattus norvegicus |
D425V | the mutant shows strongly reduced activity compared to the wild type enzyme | Rattus norvegicus |
D425W | inactive | Rattus norvegicus |
D425Y | the mutant shows strongly reduced activity compared to the wild type enzyme | Rattus norvegicus |
Q424A | the mutant shows reduced activity compared to the wild type enzyme | Rattus norvegicus |
Q426A | the mutant strongly reduced activity compared to the wild type enzyme | Rattus norvegicus |
T427A | the mutant shows reduced activity compared to the wild type enzyme | Rattus norvegicus |
Y423A | the mutant shows reduced activity compared to the wild type enzyme | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00105 | - |
L-tyrosine | mutant enzyme D425T, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.0016 | - |
L-phenylalanine | mutant enzyme D425T, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.0053 | - |
L-phenylalanine | mutant enzyme D425G, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.006 | - |
L-tyrosine | mutant enzyme D425L, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.0069 | - |
L-tyrosine | mutant enzyme D425M, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.0089 | - |
L-tyrosine | mutant enzyme D425S, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.0092 | - |
L-phenylalanine | mutant enzyme D425S, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.0105 | - |
L-phenylalanine | mutant enzyme D425Y, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.0106 | - |
L-tyrosine | mutant enzyme D425G, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.0144 | - |
L-phenylalanine | mutant enzyme D425F, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.0148 | - |
L-phenylalanine | mutant enzyme D425V, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.015 | - |
L-tyrosine | mutant enzyme D425C, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.0152 | - |
L-phenylalanine | mutant enzyme D425C, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.0164 | - |
L-phenylalanine | mutant enzyme D425M, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.0172 | - |
L-tyrosine | mutant enzyme D425F, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.0187 | - |
L-tyrosine | mutant enzyme D425H, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.019 | - |
L-phenylalanine | mutant enzyme D425L, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.021 | - |
L-phenylalanine | mutant enzyme D425A, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.022 | - |
L-phenylalanine | mutant enzyme D425I, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.028 | - |
L-phenylalanine | mutant enzyme D425R, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.03 | - |
L-tyrosine | mutant enzyme Y423A, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.0308 | - |
L-tyrosine | mutant enzyme D425Y, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.04 | - |
L-tyrosine | mutant enzyme D425V, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.04 | - |
L-tyrosine | wild type enzyme, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.041 | - |
L-tyrosine | mutant enzyme D425I, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.044 | - |
L-tyrosine | mutant enzyme Q424A, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.045 | - |
L-phenylalanine | mutant enzyme D425E, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.051 | - |
L-tyrosine | mutant enzyme T427A, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.056 | - |
L-tyrosine | mutant enzyme D425A, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.0565 | - |
L-phenylalanine | mutant enzyme D425Q, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.062 | - |
L-phenylalanine | mutant enzyme D425N, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.066 | - |
L-tyrosine | mutant enzyme Q426A, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.088 | - |
L-tyrosine | mutant enzyme D425R, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.097 | - |
L-tyrosine | mutant enzyme D425E, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.1 | - |
L-phenylalanine | wild type enzyme, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.162 | - |
L-tyrosine | mutant enzyme D425Q, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.172 | - |
L-tyrosine | mutant enzyme D425N, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
0.2 | - |
L-phenylalanine | mutant enzyme D425K, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
2.39 | - |
L-tyrosine | mutant enzyme D425K, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus | |
5 | - |
L-phenylalanine | mutant enzyme D425H, in 50 mM HEPES (pH 7.0), at 30°C | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + tetrahydrobiopterin + O2 | Rattus norvegicus | best substrate | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P04177 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + 6-methyltetrahydrobiopterin + O2 | - |
Rattus norvegicus | ? | - |
? | |
L-tryptophan + 6-methyltetrahydrobiopterin + O2 | worst substrate | Rattus norvegicus | ? | - |
? | |
L-tyrosine + 6-methyltetrahydrobiopterin + O2 | best substrate | Rattus norvegicus | 3,4-dihydroxy-L-phenylalanine + 6-methyl-4a-hydroxytetrahydrobiopterin | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | best substrate | Rattus norvegicus | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TyrH | - |
Rattus norvegicus |
tyrosine hydroxylase | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrobiopterin | - |
Rattus norvegicus |