Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.16.2 extracted from

  • Daubner, S.C.; Avila, A.; Bailey, J.O.; Barrera, D.; Bermudez, J.Y.; Giles, D.H.; Khan, C.A.; Shaheen, N.; Thompson, J.W.; Vasquez, J.; Oxley, S.P.; Fitzpatrick, P.F.
    Mutagenesis of a specificity-determining residue in tyrosine hydroxylase establishes that the enzyme is a robust phenylalanine hydroxylase but a fragile tyrosine hydroxylase (2013), Biochemistry, 52, 1446-1455.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) pLysS cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D425A the mutant shows strongly reduced activity compared to the wild type enzyme Rattus norvegicus
D425C the mutant shows strongly reduced activity compared to the wild type enzyme Rattus norvegicus
D425E the mutant shows strongly reduced activity compared to the wild type enzyme Rattus norvegicus
D425F the mutant shows strongly reduced activity compared to the wild type enzyme Rattus norvegicus
D425G the mutant shows strongly reduced activity compared to the wild type enzyme Rattus norvegicus
D425H the mutant shows strongly reduced activity compared to the wild type enzyme Rattus norvegicus
D425I the mutant shows strongly reduced activity compared to the wild type enzyme Rattus norvegicus
D425K the mutant shows strongly reduced activity compared to the wild type enzyme Rattus norvegicus
D425L the mutant shows strongly reduced activity compared to the wild type enzyme Rattus norvegicus
D425M the mutant shows strongly reduced activity compared to the wild type enzyme Rattus norvegicus
D425N the mutant shows strongly reduced activity compared to the wild type enzyme Rattus norvegicus
D425Q the mutant shows strongly reduced activity compared to the wild type enzyme Rattus norvegicus
D425R the mutant shows strongly reduced activity compared to the wild type enzyme Rattus norvegicus
D425S the mutant shows strongly reduced activity compared to the wild type enzyme Rattus norvegicus
D425T the mutant shows strongly reduced activity compared to the wild type enzyme Rattus norvegicus
D425V the mutant shows strongly reduced activity compared to the wild type enzyme Rattus norvegicus
D425W inactive Rattus norvegicus
D425Y the mutant shows strongly reduced activity compared to the wild type enzyme Rattus norvegicus
Q424A the mutant shows reduced activity compared to the wild type enzyme Rattus norvegicus
Q426A the mutant strongly reduced activity compared to the wild type enzyme Rattus norvegicus
T427A the mutant shows reduced activity compared to the wild type enzyme Rattus norvegicus
Y423A the mutant shows reduced activity compared to the wild type enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00105
-
L-tyrosine mutant enzyme D425T, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.0016
-
L-phenylalanine mutant enzyme D425T, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.0053
-
L-phenylalanine mutant enzyme D425G, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.006
-
L-tyrosine mutant enzyme D425L, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.0069
-
L-tyrosine mutant enzyme D425M, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.0089
-
L-tyrosine mutant enzyme D425S, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.0092
-
L-phenylalanine mutant enzyme D425S, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.0105
-
L-phenylalanine mutant enzyme D425Y, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.0106
-
L-tyrosine mutant enzyme D425G, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.0144
-
L-phenylalanine mutant enzyme D425F, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.0148
-
L-phenylalanine mutant enzyme D425V, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.015
-
L-tyrosine mutant enzyme D425C, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.0152
-
L-phenylalanine mutant enzyme D425C, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.0164
-
L-phenylalanine mutant enzyme D425M, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.0172
-
L-tyrosine mutant enzyme D425F, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.0187
-
L-tyrosine mutant enzyme D425H, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.019
-
L-phenylalanine mutant enzyme D425L, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.021
-
L-phenylalanine mutant enzyme D425A, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.022
-
L-phenylalanine mutant enzyme D425I, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.028
-
L-phenylalanine mutant enzyme D425R, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.03
-
L-tyrosine mutant enzyme Y423A, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.0308
-
L-tyrosine mutant enzyme D425Y, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.04
-
L-tyrosine mutant enzyme D425V, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.04
-
L-tyrosine wild type enzyme, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.041
-
L-tyrosine mutant enzyme D425I, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.044
-
L-tyrosine mutant enzyme Q424A, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.045
-
L-phenylalanine mutant enzyme D425E, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.051
-
L-tyrosine mutant enzyme T427A, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.056
-
L-tyrosine mutant enzyme D425A, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.0565
-
L-phenylalanine mutant enzyme D425Q, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.062
-
L-phenylalanine mutant enzyme D425N, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.066
-
L-tyrosine mutant enzyme Q426A, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.088
-
L-tyrosine mutant enzyme D425R, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.097
-
L-tyrosine mutant enzyme D425E, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.1
-
L-phenylalanine wild type enzyme, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.162
-
L-tyrosine mutant enzyme D425Q, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.172
-
L-tyrosine mutant enzyme D425N, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
0.2
-
L-phenylalanine mutant enzyme D425K, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
2.39
-
L-tyrosine mutant enzyme D425K, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus
5
-
L-phenylalanine mutant enzyme D425H, in 50 mM HEPES (pH 7.0), at 30°C Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tyrosine + tetrahydrobiopterin + O2 Rattus norvegicus best substrate 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P04177
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + 6-methyltetrahydrobiopterin + O2
-
Rattus norvegicus ?
-
?
L-tryptophan + 6-methyltetrahydrobiopterin + O2 worst substrate Rattus norvegicus ?
-
?
L-tyrosine + 6-methyltetrahydrobiopterin + O2 best substrate Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + 6-methyl-4a-hydroxytetrahydrobiopterin
-
?
L-tyrosine + tetrahydrobiopterin + O2 best substrate Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
?

Synonyms

Synonyms Comment Organism
TyrH
-
Rattus norvegicus
tyrosine hydroxylase
-
Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
tetrahydrobiopterin
-
Rattus norvegicus