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Literature summary for 1.14.16.2 extracted from

  • Fitzpatrick, P.F.; Ralph, E.C.; Ellis, H.R.; Willmon, O.J.; Daubner, S.C.
    Characterization of metal ligand mutants of tyrosine hydroxylase: insights into the plasticity of a 2-histidine-1-carboxylate triad (2003), Biochemistry, 42, 2081-2088.
    View publication on PubMed
Search for more literature for 1.14.16.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant enzymes H331E, H336Q, H336E, E376Q, E376H, H331E/E376H in Escherichia coli BL21 Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
E376H iron content is not significantly altered. Pterin oxidation at 1.2% of the wild-type activity. Tyrosine hydroxylation is less than 0.4% of the wild-type value Rattus norvegicus
E376Q iron content is not significantly altered. Pterin oxidation at 0.4% of the wild-type activity. Tyrosine hydroxylation is 0.39% of the wild-type value Rattus norvegicus
H331E iron content is not significantly altered Rattus norvegicus
H331E/E376H mutant enzyme contains significantly less iron than the wild-type enzyme. Pterin oxidation at 0.21% of the wild-type activity. Tyrosine hydroxylation is less than 0.4% of the wild-type value Rattus norvegicus
H331Q mutant enzyme is not successfully expressed. Pterin oxidation at 2.4% of the wild-type activity. Tyrosine hydroxylation is less than 0.002% of the wild-type value Rattus norvegicus
H336E significant decrease in iron content. Pterin oxidation at 6.3% of the wild-type activity. Tyrosine hydroxylation is 0.78% of the wild-type value Rattus norvegicus
H336Q iron-free mutant enzyme. Pterin oxidation at 11.9% of the wild-type activity. Tyrosine hydroxylation is 3.7% of the wild-type value Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.051
-
 L-Tyr pH 7.1, 25°C, wild-type enzyme Rattus norvegicus
0.051
-
 6-methyltetrahydropterin pH 7.1, 25°C, wild-type enzyme Rattus norvegicus
0.19
-
 6-methyltetrahydropterin pH 7.1, 25°C, mutant enzyme H336E Rattus norvegicus
0.311
-
 L-Tyr pH 7.1, 25°C, mutant enzyme H336Q Rattus norvegicus
0.33
-
 L-Tyr pH 7.1, 25°C, mutant enzyme H336E Rattus norvegicus
0.339
-
 6-methyltetrahydropterin pH 7.1, 25°C, mutant enzyme H331E Rattus norvegicus
0.409
-
 6-methyltetrahydropterin pH 7.1, 25°C, mutant enzyme H336Q Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + 6-methyltetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?