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Literature summary for 1.14.16.2 extracted from

  • Nasrin, S.; Ichinose, H.; Hidaka, H.; Nagatsu, T.
    Recombinant human tyrosine hydroxylase types 1-4 show regulatory kinetic properties for the natural (6R)-tetrahydrobiopterin cofactor (1994), J. Biochem., 116, 393-398.
    View publication on PubMed
Search for more literature for 1.14.16.2

Cloned(Commentary)

Cloned (Comment) Organism
4 isoforms, overexpression in Escherichia coli, phosphorylation-free Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
tyrosine substrate inhibition, with (6R)-L-erythro-tetrahydrobiopterin Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km dependent on cosubstrate type and concentration, overview for 4 isoforms Homo sapiens
0.055
-
 tyrosine recombinant isoform hTH2, with 6-methyltetrahydropterin Homo sapiens
0.066
-
 tyrosine recombinant isoform hTH4, with 6-methyltetrahydropterin Homo sapiens
0.074
-
 tyrosine recombinant isoform hTH3, with 6-methyltetrahydropterin Homo sapiens
0.166
-
 tyrosine recombinant isoform hTH1, with 6-methyltetrahydropterin Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Iron required Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 4 isoforms, gel filtration Homo sapiens
60000
-
 4 * 60000, 4 isoforms, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tyrosine + (6R)-L-erythro-tetrahydrobiopterin + O2 Homo sapiens probable regulatory role of cosubstrate for all 4 isoforms ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 4 isoforms hTH1, hTH2, hTH3, hTH4
-

Purification (Commentary)

Purification (Comment) Organism
4 isoforms, recombinant from Escherichia coli Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + (6R)-L-erythro-tetrahydrobiopterin + O2 cosubstrate has a regulatory role for all 4 isoforms Homo sapiens ?
-
 ?
L-tyrosine + (6R)-L-erythro-tetrahydrobiopterin + O2 probable regulatory role of cosubstrate for all 4 isoforms Homo sapiens ?
-
 ?
L-tyrosine + (6RS)-L-erythro-tetrahydrobiopterin + O2
-
 Homo sapiens ?
-
 ?
L-tyrosine + (6S)-L-erythro-tetrahydrobiopterin + O2
-
 Homo sapiens ?
-
 ?
L-tyrosine + 6-methyltetrahydropterin + O2
-
 Homo sapiens ?
-
 ?
L-tyrosine + tetrahydrobiopterin + O2
-
 Homo sapiens 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?

Subunits

Subunits Comment Organism
tetramer 4 * 60000, 4 isoforms, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
TH
-
 Homo sapiens
tyrosine-3-mono-oxygenase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 4 isoforms Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 4 isoforms Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 preincubation at 37°C for 2 h below, enzyme isoforms are unstable, especially isoforms hTH1 and hTH3 Homo sapiens
7
-
 preincubation at 37°C for 2 h above, isoforms are stable Homo sapiens
8
-
 isoforms, optimal stability at Homo sapiens