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Literature summary for 1.14.16.2 extracted from

  • Villanova, E.; Manjon, A.; Iborra, J.L.
    Tyrosine hydroxylase activity of immobilized tyrosinase on Enzyacryl-AA and CPG-AA supports: stabilization and properties (1984), Biotechnol. Bioeng., 26, 1306-1312.
    View publication on PubMed

Application

Application Comment Organism
synthesis immobilization of tyrosinase on polyacrylamide-based support for production of L-dopa from L-tyrosine thereby modifying the enzyme activity to tyrosine hydroxylase Pelophylax lessonae

General Stability

General Stability Organism
enzyme is stabilized by immobilization on polyacrylamide-based support Pelophylax lessonae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tyrosine + tetrahydrobiopterin + O2 Pelophylax lessonae first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
?
L-tyrosine + tetrahydrobiopterin + O2 Pelophylax lessonae reaction is coupled with ascorbate oxidation 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pelophylax lessonae
-
ridibunda, frog
-
Pelophylax lessonae
-
tyrosinase activity is modified to tyrosine hydroxylase activity via immobilization
-

Purification (Commentary)

Purification (Comment) Organism
immobilization on polyacrylamide-based support Pelophylax lessonae

Source Tissue

Source Tissue Comment Organism Textmining
epidermis
-
Pelophylax lessonae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity of immobilized enzyme under different conditions in bioreactors Pelophylax lessonae
3.46
-
purified enzyme, immobilized Pelophylax lessonae

Storage Stability

Storage Stability Organism
-30°C, immobilized and lyophilized enzyme, stored in dry environment, unaltered more than 150 days Pelophylax lessonae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + ascorbate + O2
-
Pelophylax lessonae 3,4-dihydroxy-L-phenylalanine + dehydroascorbate + H2O
-
?
L-tyrosine + tetrahydrobiopterin + O2
-
Pelophylax lessonae 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?
L-tyrosine + tetrahydrobiopterin + O2 first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine Pelophylax lessonae 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
?
L-tyrosine + tetrahydrobiopterin + O2 reaction is coupled with ascorbate oxidation Pelophylax lessonae 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
?
additional information tyrosinase activity is modified to tyrosine hydroxylase activity via immobilization Pelophylax lessonae ?
-
?

Synonyms

Synonyms Comment Organism
monophenol monooxygenase
-
Pelophylax lessonae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pelophylax lessonae