Application | Comment | Organism |
---|---|---|
synthesis | immobilization of tyrosinase on polyacrylamide-based support for production of L-dopa from L-tyrosine thereby modifying the enzyme activity to tyrosine hydroxylase | Pelophylax lessonae |
General Stability | Organism |
---|---|
enzyme is stabilized by immobilization on polyacrylamide-based support | Pelophylax lessonae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + tetrahydrobiopterin + O2 | Pelophylax lessonae | first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | Pelophylax lessonae | reaction is coupled with ascorbate oxidation | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pelophylax lessonae | - |
ridibunda, frog | - |
Pelophylax lessonae | - |
tyrosinase activity is modified to tyrosine hydroxylase activity via immobilization | - |
Purification (Comment) | Organism |
---|---|
immobilization on polyacrylamide-based support | Pelophylax lessonae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermis | - |
Pelophylax lessonae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of immobilized enzyme under different conditions in bioreactors | Pelophylax lessonae |
3.46 | - |
purified enzyme, immobilized | Pelophylax lessonae |
Storage Stability | Organism |
---|---|
-30°C, immobilized and lyophilized enzyme, stored in dry environment, unaltered more than 150 days | Pelophylax lessonae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + ascorbate + O2 | - |
Pelophylax lessonae | 3,4-dihydroxy-L-phenylalanine + dehydroascorbate + H2O | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | - |
Pelophylax lessonae | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | 3,4-dihydroxy-L-phenylalanine is identical with dopa | ? | |
L-tyrosine + tetrahydrobiopterin + O2 | first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine | Pelophylax lessonae | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | reaction is coupled with ascorbate oxidation | Pelophylax lessonae | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? | |
additional information | tyrosinase activity is modified to tyrosine hydroxylase activity via immobilization | Pelophylax lessonae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
monophenol monooxygenase | - |
Pelophylax lessonae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pelophylax lessonae |