Literature summary for 1.14.16.2 extracted from

Fitzpatrick, P.F.; Chlumsky, L.J.; Dauber, S.C.; O' Malley, K.L.
Expression of rat tyrosine hydroxylase in insect tissue culture cells and purification and characterization of the cloned enzyme (1990), J. Biol. Chem., 265, 2042-2047.

Search for more literature for 1.14.16.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Spodoptera frugiperda cells via baculovirus expression system	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.006	-	O2	below, recombinant enzyme	Rattus norvegicus
0.0094	-	tyrosine	recombinant enzyme, pH-independent	Rattus norvegicus
0.021	-	Tetrahydropterin	recombinant enzyme, value is pH-dependent	Rattus norvegicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	0.1 atom per subunit	Rattus norvegicus
phosphate	1 covalently bound residue per subunit	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	4 * 55000, recombinant enzyme, SDS-PAGE	Rattus norvegicus
250000	-	gel filtration	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
no glycoprotein	-	Rattus norvegicus

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Spodoptera frugiperda	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.03	0.1	recombinant enzyme, cell lysate	Rattus norvegicus
1.7	-	purified, recombinant enzyme	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-tyrosine + tetrahydrobiopterin + O2	-	Rattus norvegicus	3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O	3,4-dihydroxy-L-phenylalanine is identical with dopa	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 55000, recombinant enzyme, SDS-PAGE	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	broad optimum, recombinant enzyme	Rattus norvegicus
7.1	-	-	Rattus norvegicus

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Release 2023.1 (January 2023)