Activating Compound | Comment | Organism | Structure |
---|---|---|---|
heparin | 0.1 mg/ml, activates | Rattus norvegicus | |
additional information | activation by phosphorylation | Rattus norvegicus | |
phosphatidylinositol | 0.1 mg/ml activates | Rattus norvegicus | |
SDS | 0.01%, activates | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | phosphorylation converts the enzyme from a form possessing a high Km for pterin cofactor to a form with a low Km for pterin cofactor | Rattus norvegicus | |
additional information | - |
additional information | rat brain: 2 kinetically distinguishable forms: low Km form, high Km form | Rattus norvegicus | |
0.075 | - |
tyrosine | with 6-methyltetrahydropterin | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
4 * 56000, about, pheochromocytoma tumor, SDS-PAGE and amino acid sequence analysis | Rattus norvegicus |
225000 | - |
pheochromocytoma tumors, asymmetric protein, native PAGE, gel filtration and sucrose density gradient centrifugation | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + (6R)-L-erythro-1',2'-dihydroxypropyltetrahydropterin + O2 | Rattus norvegicus | first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine | ? | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | Rattus norvegicus | first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pheochromocytoma cell | tumor | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
330 | - |
purified enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + (6R)-L-erythro-1',2'-dihydroxypropyltetrahydropterin + O2 | first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine | Rattus norvegicus | ? | - |
? | |
L-tyrosine + 2-amino-4-hydroxy-6,7-dimethyltetrahydropterin + O2 | synthetic pterin as electron donor | Rattus norvegicus | ? | - |
? | |
L-tyrosine + 2-amino-4-hydroxy-6-methyltetrahydropterin + O2 | synthetic pterin as electron donor | Rattus norvegicus | ? | - |
? | |
L-tyrosine + 6-methyltetrahydropterin + O2 | - |
Rattus norvegicus | ? | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | 3,4-dihydroxy-L-phenylalanine is identical with dopa | ? | |
L-tyrosine + tetrahydrobiopterin + O2 | first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine | Rattus norvegicus | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 56000, about, pheochromocytoma tumor, SDS-PAGE and amino acid sequence analysis | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Rattus norvegicus |