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Literature summary for 1.14.16.1 extracted from

  • Zhang, S.; Fitzpatrick, P.F.
    Identification of the allosteric site for phenylalanine in rat phenylalanine hydroxylase (2016), J. Biol. Chem., 291, 7418-7425 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.16.1

Activating Compound

Activating Compound Comment Organism Structure
L-phenylalanine
-
 Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
A47G the mutation decreases the affinity of the catalytic domain for L-phenylalanine compared to the wild type Rattus norvegicus
E44Q the mutation decreases the affinity of the catalytic domain for L-phenylalanine compared to the wild type and leads to loss of activity Rattus norvegicus
H64N the mutation decreases the affinity of the catalytic domain for L-phenylalanine compared to the wild type Rattus norvegicus
L48V the mutation decreases the affinity of the catalytic domain for L-phenylalanine compared to the wild type Rattus norvegicus
L62V the mutation decreases the affinity of the catalytic domain for L-phenylalanine compared to the wild type and leads to loss of activity Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-phenylalanine + tetrahydrobiopterin + O2 Rattus norvegicus
-
 L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P04176
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydrobiopterin + O2
-
 Rattus norvegicus L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?

Synonyms

Synonyms Comment Organism
PheH
-
 Rattus norvegicus
phenylalanine hydroxylase
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2
-
 L-phenylalanine mutant enzyme L48V, at pH 7.0 and 23°C Rattus norvegicus
5.8
-
 L-phenylalanine mutant enzyme A47G, at pH 7.0 and 23°C Rattus norvegicus
6.5
-
 L-phenylalanine mutant enzyme H64N, at pH 7.0 and 23°C Rattus norvegicus
9.7
-
 L-phenylalanine wild type enzyme, at pH 7.0 and 23°C Rattus norvegicus