Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.16.1 extracted from

  • Flydal, M.I.; Chatfield, C.H.; Zheng, H.; Gunderson, F.F.; Aubi, O.; Cianciotto, N.P.; Martinez, A.
    Phenylalanine hydroxylase from Legionella pneumophila is a thermostable enzyme with a major functional role in pyomelanin synthesis (2012), PLoS ONE, 7, e46209.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.16.1

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is not affected in any direction by preincubation with the substrate L-phenylalanine Legionella pneumophila

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Legionella pneumophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.125
-
 tetrahydrobiopterin in 100 mM Na-HEPES, pH 7.0 at 37°C Legionella pneumophila
0.735
-
 L-phenylalanine in 100 mM Na-HEPES, pH 7.0 at 37°C Legionella pneumophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 2 * 31000, SDS-PAGE Legionella pneumophila
32500
-
 2 * 32500, calculated from amino acid sequence Legionella pneumophila
64000
-
 gel filtration Legionella pneumophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-phenylalanine + tetrahydrobiopterin + O2 Legionella pneumophila
-
 L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
L-phenylalanine + tetrahydrobiopterin + O2 Legionella pneumophila 130b
-
 L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?

Organism

Organism UniProt Comment Textmining
Legionella pneumophila
-
-
-
Legionella pneumophila 130b
-
-
-

Purification (Commentary)

Purification (Comment) Organism
TALON resin column chromatography and Superdex 200 gel filtration Legionella pneumophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.2
-
 in 100 mM Na-HEPES, pH 7.0 at 37°C Legionella pneumophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydrobiopterin + O2
-
 Legionella pneumophila L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
L-phenylalanine + tetrahydrobiopterin + O2
-
 Legionella pneumophila 130b L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 31000, SDS-PAGE Legionella pneumophila
homodimer 2 * 32500, calculated from amino acid sequence Legionella pneumophila

Synonyms

Synonyms Comment Organism
PAH
-
 Legionella pneumophila
phenylalanine hydroxylase
-
 Legionella pneumophila
phhA
-
 Legionella pneumophila

General Information

General Information Comment Organism
physiological function the enzyme has a major functional role in the synthesis of pyomelanin and promotes growth in media lacking tyrosine Legionella pneumophila