Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Caenorhabditis elegans |
Protein Variants | Comment | Organism |
---|---|---|
Q215K/N216Y | humanized mutant Q215K/N216Y of cePAH binds 1.4 L-Phe/subunit. This mutant also displays high catalytic activity and certain positive cooperativity for L-Phe. Km for cofactor tetrahydrobiopterin higher compared to wild-type, [S]0.5 (L-Phe) lower compared to wild-type, Vmax (L-Phe) higher compared to wild-type | Caenorhabditis elegans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | [S]0.5 (L-phenylalanine): 0.188 mM wild-type,0,109 mM mutant Q215K/N216Y, Vmax (wild-type): 1.9 micromol L-Tyr/min/mg, Vmax (mutant Q215K/N216Y): 6.6 micromol L-Tyr/min/mg, pH 7.0, 25°C | Caenorhabditis elegans | |
0.029 | - |
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin | wild-type, pH 7.0, 25°C | Caenorhabditis elegans | |
0.094 | - |
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin | mutant Q215K/N216Y, pH 7.0, 25°C | Caenorhabditis elegans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-phenylalanine + a 5,6,7,8-tetrahydropteridine + O2 = L-tyrosine + a 4a-hydroxy-5,6,7,8-tetrahydropteridine | structural analysis reveal an additional L-Phe binding site at the regulatory domain of full-length cePAH. This site involves the GA(S)L/ISRP motifs, which are also found in ACT domains of other L-Phe binding proteins | Caenorhabditis elegans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.1 | - |
micromol L-Tyr/min/mg, wild-type, pH 7.0, 25°C, without L-Phe preincubated enzyme | Caenorhabditis elegans |
2.6 | - |
micromol L-Tyr/min/mg, wild-type, pH 7.0, 25°C, with L-Phe preincubated enzyme | Caenorhabditis elegans |
4.9 | - |
micromol L-Tyr/min/mg, mutant Q215K/N216Y, pH 7.0, 25°C, with L-Phe preincubated enzyme | Caenorhabditis elegans |
6.5 | - |
micromol L-Tyr/min/mg, mutant Q215K/N216Y, pH 7.0, 25°C, with L-Phe preincubated enzyme | Caenorhabditis elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + O2 | - |
Caenorhabditis elegans | L-tyrosine + 4a-hydroxy-(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Caenorhabditis elegans |
Synonyms | Comment | Organism |
---|---|---|
cePAH | - |
Caenorhabditis elegans |
phenylalanine hydroxylase | - |
Caenorhabditis elegans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Caenorhabditis elegans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Caenorhabditis elegans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrobiopterin | - |
Caenorhabditis elegans |