Literature summary for 1.14.16.1 extracted from

Patel, N.; Iliadou, C.; Boonyapiwat, B.; Barlow, D.; Forbes, B.; Mitchell, S.; Steventon, G.
Enzyme kinetic and molecular modelling studies of sulphur-containing substrates of phenylalanine 4-monooxygenase (2008), J. Enzyme Inhib. Med. Chem., 23, 958-963.

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Inhibitors

Inhibitors	Comment	Organism	Structure
L-methionine	competitive inhibitor of the C-oxidation of L-phenylalanine	Homo sapiens
L-methionine	competitive inhibitor of the C-oxidation of L-phenylalanine	Rattus norvegicus
S-carboxy-methyl-L-cysteine	competitive inhibitor of the C-oxidation of L-phenylalanine	Homo sapiens
S-carboxy-methyl-L-cysteine	competitive inhibitor of the C-oxidation of L-phenylalanine	Rattus norvegicus
S-methyl-ergothionine	competitive inhibitor of the C-oxidation of L-phenylalanine	Homo sapiens
S-methyl-ergothionine	competitive inhibitor of the C-oxidation of L-phenylalanine	Rattus norvegicus
S-methyl-L-cysteine	competitive inhibitor of the C-oxidation of L-phenylalanine	Homo sapiens
S-methyl-L-cysteine	competitive inhibitor of the C-oxidation of L-phenylalanine	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.26	-	L-phenylalanine	at 37°C, 50 mM potassium phosphate buffer, pH 6.8	Rattus norvegicus
0.3	-	S-methyl-ergothionine	at 37°C, 50 mM potassium phosphate buffer, pH 6.8	Homo sapiens
0.45	-	S-methyl-ergothionine	at 37°C, 50 mM potassium phosphate buffer, pH 6.8	Rattus norvegicus
0.64	-	L-phenylalanine	at 37°C, 50 mM potassium phosphate buffer, pH 6.8	Homo sapiens
3.1	-	L-methionine	at 37°C, 50 mM potassium phosphate buffer, pH 6.8	Homo sapiens
4.6	-	S-carboxy-methyl-L-cysteine	at 37°C, 50 mM potassium phosphate buffer, pH 6.8	Homo sapiens
7.75	-	L-methionine	at 37°C, 50 mM potassium phosphate buffer, pH 6.8	Rattus norvegicus
14.73	-	S-carboxy-methyl-L-cysteine	at 37°C, 50 mM potassium phosphate buffer, pH 6.8	Rattus norvegicus
18.32	-	S-methyl-L-cysteine	at 37°C, 50 mM potassium phosphate buffer, pH 6.8	Homo sapiens
43.25	-	S-methyl-L-cysteine	at 37°C, 50 mM potassium phosphate buffer, pH 6.8	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Homo sapiens	5829	-
cytosol	-	Rattus norvegicus	5829	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Rattus norvegicus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Homo sapiens	-
liver	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-methionine + 5,6,7,8-tetrahydrobiopterin + O2	-	Homo sapiens	?	-	?
L-methionine + 5,6,7,8-tetrahydrobiopterin + O2	-	Rattus norvegicus	?	-	?
L-phenylalanine + 5,6,7,8-tetrahydrobiopterin + O2	-	Homo sapiens	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?
L-phenylalanine + 5,6,7,8-tetrahydrobiopterin + O2	-	Rattus norvegicus	L-tyrosine + 4a-hydroxytetrahydrobiopterin	-	?
additional information	the rate of clearance of the substrates via human PAH is L-phenylalanine > L-methionine > S-carboxy-methyl-L-cysteine > S-methyl-L-cysteine	Homo sapiens	?	-	?
additional information	the rate of clearance of the substrates via rat PAH is L-phenylalanine > L-methionine > S-carboxy-methyl-L-cysteine > S-methyl-L-cysteine	Rattus norvegicus	?	-	?
S-carboxy-methyl-L-cysteine + 5,6,7,8-tetrahydrobiopterin + O2	poor substrate	Homo sapiens	S-carboxymethyl-L-cysteine S-oxide + dihydrobiopterin + H2O	-	?
S-carboxy-methyl-L-cysteine + 5,6,7,8-tetrahydrobiopterin + O2	poor substrate	Rattus norvegicus	S-carboxymethyl-L-cysteine S-oxide + dihydrobiopterin + H2O	-	?
S-methyl-ergothionine + 5,6,7,8-tetrahydrobiopterin + O2	-	Homo sapiens	?	-	?
S-methyl-ergothionine + 5,6,7,8-tetrahydrobiopterin + O2	-	Rattus norvegicus	?	-	?
S-methyl-L-cysteine + 5,6,7,8-tetrahydrobiopterin + O2	poor substrate	Homo sapiens	S-methyl-L-cysteine S-oxide + dihydrobiopterin + H2O	-	?
S-methyl-L-cysteine + 5,6,7,8-tetrahydrobiopterin + O2	poor substrate	Rattus norvegicus	S-methyl-L-cysteine S-oxide + dihydrobiopterin + H2O	-	?

Synonyms

Synonyms	Comment	Organism
PAH	-	Homo sapiens
PAH	-	Rattus norvegicus
phenylalanine hydroxylase	-	Homo sapiens
phenylalanine hydroxylase	-	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
5,6,7,8-tetrahydro-L-biopterin	-	Homo sapiens
5,6,7,8-tetrahydro-L-biopterin	-	Rattus norvegicus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.41	-	S-methyl-ergothionine	at 37°C, 50 mM potassium phosphate buffer, pH 6.8	Homo sapiens
0.5	-	S-methyl-ergothionine	at 37°C, 50 mM potassium phosphate buffer, pH 6.8	Rattus norvegicus
3.5	-	L-methionine	at 37°C, 50 mM potassium phosphate buffer, pH 6.8	Homo sapiens
5.01	-	S-carboxy-methyl-L-cysteine	at 37°C, 50 mM potassium phosphate buffer, pH 6.8	Homo sapiens
8.11	-	L-methionine	at 37°C, 50 mM potassium phosphate buffer, pH 6.8	Rattus norvegicus
13.14	-	S-carboxy-methyl-L-cysteine	at 37°C, 50 mM potassium phosphate buffer, pH 6.8	Rattus norvegicus
17.32	-	S-methyl-L-cysteine	at 37°C, 50 mM potassium phosphate buffer, pH 6.8	Homo sapiens
41.53	-	S-methyl-L-cysteine	at 37°C, 50 mM potassium phosphate buffer, pH 6.8	Rattus norvegicus

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Release 2023.1 (January 2023)