Cloned (Comment) | Organism |
---|---|
expression of catalytic core mutant DELTA 117PheH and point mutants thereof in Escherichia coli strain C41(DE3) | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
E280A | site-directed mutagenesis of catalytic core mutant DELTA117PheH, 70% reduced activity but unaltered isotopic effects of isotope substrates | Rattus norvegicus |
F263A | site-directed mutagenesis of catalytic core mutant DELTA117PheH, 85% reduced activity but unaltered isotopic effects of isotope substrates | Rattus norvegicus |
additional information | construction and catalytic properties of deletion mutant DELTA117PheH, consisting of the catalytic core of the enzyme, the isotopic effects of substrates [4-2H]-, [3,5-2H2]-, and 2H5-phenylalanine are unaltered compared to the wild-type enzyme, overview | Rattus norvegicus |
V379D | site-directed mutagenesis of catalytic core mutant DELTA117PheH, the isotopic effects of substrates [4-2H]-, [3,5-2H2]-, and 2H5-phenylalanine are altered compared to the wild-type enzyme, overview | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
6-methyl-tetrahydrobiopterin | pH 7.0, 25°C, recombinant mutant DELTA 117PheH V379D | Rattus norvegicus | |
0.083 | - |
6-methyl-tetrahydrobiopterin | pH 7.0, 25°C, recombinant mutant DELTA 117PheH | Rattus norvegicus | |
0.12 | - |
L-phenylalanine | pH 7.0, 25°C, with cofactor 6-methyl-tetrahydrobiopterin, recombinant mutant DELTA 117PheH | Rattus norvegicus | |
0.42 | - |
L-phenylalanine | pH 7.0, 25°C, with cofactor 6-methyl-tetrahydrobiopterin, recombinant mutant DELTA 117PheH V379D | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + 5,6,7,8-tetrahydrobiopterin + O2 | Rattus norvegicus | - |
L-tyrosine + 4a-hydroxy-tetrahydrobiopterin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant catalytic core mutant DELTA 117PheH and point mutants thereof from Escherichia coli strain C41(DE3) | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-phenylalanine + a 5,6,7,8-tetrahydropteridine + O2 = L-tyrosine + a 4a-hydroxy-5,6,7,8-tetrahydropteridine | catalytic mechanism | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + 5,6,7,8-tetrahydrobiopterin + O2 | - |
Rattus norvegicus | L-tyrosine + 4a-hydroxy-tetrahydrobiopterin | - |
? | |
L-phenylalanine + 5,6,7,8-tetrahydrobiopterin + O2 | specific for the L-isomer, no activity with the D-isomer, effects of isotopic substrates [4-2H]-, [3,5-2H2]-, and 2H5-phenylalanine, overview | Rattus norvegicus | L-tyrosine + 4a-hydroxy-tetrahydrobiopterin | - |
? | |
L-phenylalanine + 6-methyl-tetrahydrobiopterin + O2 | - |
Rattus norvegicus | L-tyrosine + 6-methyl-4-hydroxy-tetrahydrobiopterin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PheH | - |
Rattus norvegicus |
phenylalanine hydroxylase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | 0.51 | 6-methyl-tetrahydrobiopterin | pH 7.0, 25°C, recombinant mutant DELTA 117PheH | Rattus norvegicus | |
0.04 | 1.97 | L-phenylalanine | pH 7.0, 25°C, with cofactor 6-methyl-tetrahydrobiopterin, recombinant mutant DELTA 117PheH | Rattus norvegicus | |
0.3 | - |
L-phenylalanine | pH 7.0, 25°C, with cofactor 6-methyl-tetrahydrobiopterin, recombinant mutant DELTA 117PheH V379D | Rattus norvegicus | |
1.12 | - |
6-methyl-tetrahydrobiopterin | pH 7.0, 25°C, recombinant mutant DELTA 117PheH V379D | Rattus norvegicus | |
7.85 | - |
6-methyl-tetrahydrobiopterin | pH 7.0, 25°C, recombinant mutant DELTA 117PheH | Rattus norvegicus | |
8.55 | - |
L-phenylalanine | pH 7.0, 25°C, with cofactor 6-methyl-tetrahydrobiopterin, recombinant mutant DELTA 117PheH | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6,7,8-tetrahydrobiopterin | - |
Rattus norvegicus | |
6-methyl-tetrahydrobiopterin | - |
Rattus norvegicus |