Literature summary for 1.14.16.1 extracted from

Erlandsen, H.; Kim, J.Y.; Patch, M.G.; Han, A.; Volner, A.; Abu-Omar, M.M.; Stevens, R.C.
Structural comparison of bacterial and human iron-dependent phenylalanine hydroxylases: similar fold, different stability and reaction rates (2002), J. Mol. Biol., 320, 645-661.

Search for more literature for 1.14.16.1

Cloned(Commentary)

Cloned (Comment)	Organism
-	Chromobacterium violaceum

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapor-diffusion hanging drop method at 4°C, reservoir solution contains 1 ml of 1.65-1.9 M ammonium sulfate, 40-100 mM NaCl and 20 mM HEPES pH 7.5, hanging drops are made using equal volumes of enzyme, 20 mg/ml, and reservoir solution, crystals grow in about one week, crystal structures of Fe-free apoenzyme, Fe3+-bound enzyme and Fe3+ plus 7,8-dihydro-L-biopterin-bound enzyme at 1.7 A, 2.0 A and 1.4 A resolution respectively	Chromobacterium violaceum

Crystallization (Comment)

Organism

vapor-diffusion hanging drop method at 4°C, reservoir solution contains 1 ml of 1.65-1.9 M ammonium sulfate, 40-100 mM NaCl and 20 mM HEPES pH 7.5, hanging drops are made using equal volumes of enzyme, 20 mg/ml, and reservoir solution, crystals grow in about one week, crystal structures of Fe-free apoenzyme, Fe3+-bound enzyme and Fe3+ plus 7,8-dihydro-L-biopterin-bound enzyme at 1.7 A, 2.0 A and 1.4 A resolution respectively

Chromobacterium violaceum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	-	Homo sapiens
Iron	enzyme is active only in the presence of Fe2+	Chromobacterium violaceum

Organism

Organism	UniProt	Comment	Textmining
Chromobacterium violaceum	P30967	-	-
Homo sapiens	P00439	-	-

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)