Cloned (Comment) | Organism |
---|---|
expression of wild-type, R270K and V388M mutant enzymes in the presence of the chemical chaperone glycerol | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R270K | expression in the presence of the chemical chaperone glycerol enhances activity after purification | Homo sapiens |
V388M | expression in the presence of the chemical chaperone glycerol enhances activity after purification | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00439 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type, R270K and V388M mutant enzymes expressed in the presence and absence of glycerol | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.099 | - |
R270K mutant enzyme, expression in the absence of glycerol in the growth medium, cofactor 6-methyltetrahydropterin | Homo sapiens |
0.23 | - |
R270K mutant enzyme, expression in the presence of glycerol in the growth medium, cofactor 6-methyltetrahydropterin | Homo sapiens |
1.773 | - |
V388M mutant enzyme, expression in the absence of glycerol in the growth medium, cofactor 6-methyltetrahydropterin | Homo sapiens |
2.91 | - |
V388M mutant enzyme, expression in the presence of glycerol in the growth medium, cofactor 6-methyltetrahydropterin | Homo sapiens |