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Literature summary for 1.14.16.1 extracted from
- Phenylalanine hydroxylase from Chromobacterium violaceum. Uncoupled oxidation of tetrahydropterin and the role of iron in hydroxylation (1998), J. Biol. Chem., 273, 25594-25601.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Chromobacterium violaceum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | substoichiometric amounts after removal of copper with dithiothreitol | Chromobacterium violaceum |  |
| copper | copper does not support enzyme activity and can be removed by dithiothreitol | Chromobacterium violaceum | |
| Iron | copper depleted enzyme can be reconstituted with approx. 1 molecule of iron per enzyme molecule, iron reconstituted enzyme hydroxylates phenylalanine | Chromobacterium violaceum | |
| Zn2+ | substoichiometric amounts after removal of copper with dithiothreitol | Chromobacterium violaceum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33613 | - |
1 * 33613, deduced from nucleotide sequence | Chromobacterium violaceum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chromobacterium violaceum | - |
most probably 2 phenylalanine hydroxylases encoded by different genes | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-phenylalanine + 6-methyltetrahydropterin + O2 | - |
Chromobacterium violaceum | L-tyrosine + 4a-hydroxy-6-methyltetrahydropterin | in the presence of FeSO4 and dithiothreitol | ? | |
| L-phenylalanine + 6-methyltetrahydropterin + O2 | copper-depleted enzyme, in the absence of Fe2+, 6-methyltetrahydropterin oxidation can be uncoupled from substrate hydroxylation by the exclusion of iron | Chromobacterium violaceum | L-tyrosine + 4a-hydroxy-6-methyltetrahydropterin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33483-33488, recombinant enzyme, mass spectrometry | Chromobacterium violaceum |
| monomer | 1 * 33613, deduced from nucleotide sequence | Chromobacterium violaceum |
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Release 2023.1 (January 2023)
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