Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cAMP-dependent protein kinase | - |
Rattus norvegicus | |
cAMP-dependent protein kinase | 1.5fold stimulation of recombinant enzyme | Homo sapiens | |
lysolecithin | - |
Rattus norvegicus | |
lysolecithin | 2fold activation of recombinant enzyme, cofactor tetrahydrobiopterin | Homo sapiens | |
phenylalanine | - |
Rattus norvegicus | |
phenylalanine | recombinant enzyme, 2fold increase in activity, cofactor tetrahydrobiopterin | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(7R)-5,6,7,8-tetrahydrobiopterin | - |
Homo sapiens | |
(7R)-5,6,7,8-tetrahydropterin | 0.001 mM, 50% inhibition at 0.5 mM phenylalanine, 0.004 mM, 50% inhibition at 0.1 mM phenylalanine, recombinant enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
(6R)-5,6,7,8-tetrahydrobiopterin | recombinant enzyme | Homo sapiens | |
0.025 | - |
(6R)-5,6,7,8-tetrahydrobiopterin | recombinant enzyme, phenylalanine-activated | Homo sapiens | |
0.043 | - |
phenylalanine | recombinant enzyme, cofactor (6R)-methyltetrahydropterin | Homo sapiens | |
0.045 | - |
6-methyltetrahydropterin | recombinant enzyme | Homo sapiens | |
0.049 | - |
phenylalanine | phenylalanine-activated recombinant enzyme, cofactor tetrahydrobiopterin | Homo sapiens | |
0.05 | - |
phenylalanine | recombinant enzyme, cofactor tetrahydrobiopterin | Homo sapiens | |
0.055 | - |
phenylalanine | recombinant enzyme, cofactor (7R)-5,6,7,8-tetrahydrobiopterin | Homo sapiens | |
0.2 | - |
(7R)-5,6,7,8-tetrahydrobiopterin | recombinant enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | incorporation of 0.6 mol phosphate per enzyme subunit, recombinant enzyme | Homo sapiens |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Phenyl-Sepharose, DEAE-Sepharose, recombinant enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.6 | - |
recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + (7R)-5,6,7,8-tetrahydrobiopterin + O2 | - |
Homo sapiens | L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
r |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
(7R)-5,6,7,8-tetrahydrobiopterin | - |
Homo sapiens |