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Literature summary for 1.14.16.1 extracted from
- The isolation and properties of phenylalanine hydroxylase from human liver (1974), Biochem. J., 139, 741-749.
General Stability
| General Stability | Organism |
|---|---|
| phenylalanine stabilizes | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | 1 mM, complete inhibition after 10 min | Homo sapiens |  |
| 4-Chlorophenylalanine | competitive vs. phenylalanine | Homo sapiens | |
| 4-Fluorophenylalanine | above 1 mM | Homo sapiens | |
| copper-chelating agents | - |
Homo sapiens | |
| diethyldithiocarbamate | 1 mM, 58% inhibition | Homo sapiens | |
| DL-alpha-tocopherol | strong inhibition | Homo sapiens | |
| Iron-chelating agents | - |
Homo sapiens | |
| Thiol-binding reagents | - |
Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.318 | - |
L-phenylalanine | - |
Homo sapiens | |
| 1 | - |
4-Fluorophenylalanine | approx. value | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
2 * 54000, fetal liver enzyme, SDS-PAGE | Homo sapiens |
| 110000 | - |
fetal liver enzyme, gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| fetal, newborn and adult enzyme are probably identical | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.225 | - |
liver enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-fluorophenylalanine + tetrahydrobiopterin + O2 | - |
Homo sapiens | ? | - |
? | |
| L-phenylalanine + tetrahydrobiopterin + O2 | - |
Homo sapiens | L-tyrosine + dihydrobiopterin + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 54000, fetal liver enzyme, SDS-PAGE | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| tetrahydrobiopterin | - |
Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
4-Chlorophenylalanine | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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