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Literature summary for 1.14.16.1 extracted from

  • Abita, J.P.; Parniak, M.; Kaufman, S.
    The activation of rat liver phenylalanine hydroxylase by limited proteolysis, lysolecithin, and tocopherol phosphate. Changes in conformation and catalytic properties (1984), J. Biol. Chem., 259, 14560-14566.
    View publication on PubMed
Search for more literature for 1.14.16.1

Activating Compound

Activating Compound Comment Organism Structure
alpha-chymotrypsin limited proteolysis of purified liver enzyme, 20-30fold increase in activity, cofactor tetrahydrobiopterin Rattus norvegicus
liver lysosomal proteases limited proteolysis of liver enzyme Rattus norvegicus
lysolecithin
-
 Rattus norvegicus
additional information relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis Rattus norvegicus
Phospholipids activate Rattus norvegicus
Phospholipids increase in activity in the presence of tetrahydrobiopterin but not in the presence of synthetic pterin cofactors Rattus norvegicus
Trypsin limited proteolysis of purified liver enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 tetrahydrobiopterin native liver enzyme Rattus norvegicus
0.012
-
 tetrahydrobiopterin chymotrypsin activated liver enzyme Rattus norvegicus
0.045
-
 6-methyltetrahydropterin native liver enzyme Rattus norvegicus
0.065
-
 6,7-dimethyltetrahydrobiopterin native liver enzyme Rattus norvegicus
0.065
-
 6,7-dimethyltetrahydrobiopterin native enzyme, at pH 6.8 and 25°C Rattus norvegicus
0.087
-
 6-methyltetrahydropterin chymotrypsin activated liver enzyme Rattus norvegicus
0.105
-
 6,7-dimethyltetrahydrobiopterin chymotrypsin activated liver enzyme Rattus norvegicus
0.105
-
 6,7-dimethyltetrahydrobiopterin chymotrypsin-activated enzyme, at pH 6.8 and 25°C Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + 6,7-dimethyltetrahydrobiopterin
-
 Rattus norvegicus L-tyrosine + 6,7-dimethyl-4a-hydroxy-tetrahydrobiopterin
-
 r
L-phenylalanine + tetrahydrobiopterin + O2 relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis Rattus norvegicus L-tyrosine + dihydrobiopterin + H2O
-
 ?
L-phenylalanine + tetrahydrobiopterin + O2 low activity with tetrahydrobiopterin can be selectively increased by a wide variety of reversible and irreversible modificators of the enzyme, e.g. interaction with phospholipids Rattus norvegicus L-tyrosine + dihydrobiopterin + H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
tetrahydrobiopterin
-
 Rattus norvegicus