Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-chymotrypsin | limited proteolysis of purified liver enzyme, 20-30fold increase in activity, cofactor tetrahydrobiopterin | Rattus norvegicus | |
liver lysosomal proteases | limited proteolysis of liver enzyme | Rattus norvegicus | |
lysolecithin | - |
Rattus norvegicus | |
additional information | relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis | Rattus norvegicus | |
Phospholipids | activate | Rattus norvegicus | |
Phospholipids | increase in activity in the presence of tetrahydrobiopterin but not in the presence of synthetic pterin cofactors | Rattus norvegicus | |
Trypsin | limited proteolysis of purified liver enzyme | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
tetrahydrobiopterin | native liver enzyme | Rattus norvegicus | |
0.012 | - |
tetrahydrobiopterin | chymotrypsin activated liver enzyme | Rattus norvegicus | |
0.045 | - |
6-methyltetrahydropterin | native liver enzyme | Rattus norvegicus | |
0.065 | - |
6,7-dimethyltetrahydrobiopterin | native liver enzyme | Rattus norvegicus | |
0.065 | - |
6,7-dimethyltetrahydrobiopterin | native enzyme, at pH 6.8 and 25°C | Rattus norvegicus | |
0.087 | - |
6-methyltetrahydropterin | chymotrypsin activated liver enzyme | Rattus norvegicus | |
0.105 | - |
6,7-dimethyltetrahydrobiopterin | chymotrypsin activated liver enzyme | Rattus norvegicus | |
0.105 | - |
6,7-dimethyltetrahydrobiopterin | chymotrypsin-activated enzyme, at pH 6.8 and 25°C | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + 6,7-dimethyltetrahydrobiopterin | - |
Rattus norvegicus | L-tyrosine + 6,7-dimethyl-4a-hydroxy-tetrahydrobiopterin | - |
r | |
L-phenylalanine + tetrahydrobiopterin + O2 | relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis | Rattus norvegicus | L-tyrosine + dihydrobiopterin + H2O | - |
? | |
L-phenylalanine + tetrahydrobiopterin + O2 | low activity with tetrahydrobiopterin can be selectively increased by a wide variety of reversible and irreversible modificators of the enzyme, e.g. interaction with phospholipids | Rattus norvegicus | L-tyrosine + dihydrobiopterin + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrobiopterin | - |
Rattus norvegicus |