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Literature summary for 1.14.16.1 extracted from

  • Pember, S.O.; Villafranca, J.J.; Benkovic, S.J.
    Chromobacterium violaceum phenylalanine 4-monooxygenase (1987), Methods Enzymol., 142, 50-56.
    View publication on PubMed
Search for more literature for 1.14.16.1

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
 Chromobacterium violaceum

Inhibitors

Inhibitors Comment Organism Structure
Bathocuproine
-
 Chromobacterium violaceum
citrate
-
 Chromobacterium violaceum
diethyldithiocarbamate
-
 Chromobacterium violaceum
dopamine
-
 Chromobacterium violaceum
EDTA
-
 Chromobacterium violaceum
o-phenanthroline
-
 Chromobacterium violaceum
phosphate
-
 Chromobacterium violaceum

Metals/Ions

Metals/Ions Comment Organism Structure
copper 1 mol of Cu2+ per mol of enzyme Chromobacterium violaceum
additional information enzyme does not contain iron Chromobacterium violaceum

Organism

Organism UniProt Comment Textmining
Chromobacterium violaceum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Chromobacterium violaceum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.9
-
-
 Chromobacterium violaceum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydrobiopterin + O2 additional electron donors: 6-methylpterin, 6,7-dimethyltetrahydropterin Chromobacterium violaceum L-tyrosine + dihydrobiopterin + H2O
-
 ?
L-phenylalanine + tetrahydrobiopterin + O2 additional electron donors: 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine, 2-amino-4-hydroxy-6-methyltetrahydropteridine, tetrahydrofolate, and 6-methyl-5-deazatetrahydropterin Chromobacterium violaceum L-tyrosine + dihydrobiopterin + H2O
-
 ?
L-tryptophan + tetrahydrobiopterin + O2 0.4% of activity with L-phenylalanine Chromobacterium violaceum ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
additional information artificial cofactors: 6-methyltetrahydropterin, 6,7-dimethyltetrahydropterin Chromobacterium violaceum
additional information artificial cofactors: 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine, 6-methyl-5-deazatetrahydropterin and 2-amino-4-hydroxy-6-methyltetrahydropteridine can replace tetrahydrobiopterin Chromobacterium violaceum
tetrahydrobiopterin
-
 Chromobacterium violaceum