Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cAMP-dependent protein kinase | - |
Homo sapiens | |
cAMP-dependent protein kinase | - |
Rattus norvegicus | |
lysolecithin | - |
Homo sapiens | |
lysolecithin | - |
Rattus norvegicus | |
additional information | relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis | Homo sapiens | |
additional information | relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis | Rattus norvegicus | |
additional information | enzyme activity is not stimulated by phosphorylation | Homo sapiens | |
Phospholipids | activate | Homo sapiens | |
Phospholipids | activate | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
tetrahydrobiopterin | - |
Homo sapiens | |
0.033 | - |
6,7-dimethyltetrahydropterin | - |
Homo sapiens | |
0.05 | - |
phenylalanine | cofactor tetrahydrobiopterin | Homo sapiens | |
0.55 | - |
phenylalanine | cofactor dimethyltetrahydropterin | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
2 * 54000, fetal liver enzyme, SDS-PAGE | Homo sapiens |
107000 | - |
fetal liver enzyme, sucrose density gradient centrifugation | Homo sapiens |
165000 | - |
gel filtration | Homo sapiens |
275000 | - |
- |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | - |
Homo sapiens |
side-chain modification | enzyme contains 0.3 mol of phosphate per mol of enzyme subunit | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
Phenyl-Sepharose, DEAE-Sepharose | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.106 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C, enzyme concentration 1 mg/ml, several months, no loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + 6,7-dimethyltetrahydrobiopterin | - |
Homo sapiens | L-tyrosine + 6,7-dimethyl-4a-hydroxy-tetrahydrobiopterin | - |
r | |
L-phenylalanine + tetrahydrobiopterin + O2 | relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis | Homo sapiens | L-tyrosine + dihydrobiopterin + H2O | - |
? | |
L-phenylalanine + tetrahydrobiopterin + O2 | relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis | Rattus norvegicus | L-tyrosine + dihydrobiopterin + H2O | - |
? | |
L-phenylalanine + tetrahydrobiopterin + O2 | low activity with tetrahydrobiopterin can be selectively increased by a wide variety of reversible and irreversible modificators of the enzyme, e.g. interaction with phospholipids | Homo sapiens | L-tyrosine + dihydrobiopterin + H2O | - |
? | |
L-phenylalanine + tetrahydrobiopterin + O2 | low activity with tetrahydrobiopterin can be selectively increased by a wide variety of reversible and irreversible modificators of the enzyme, e.g. interaction with phospholipids | Rattus norvegicus | L-tyrosine + dihydrobiopterin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Rattus norvegicus |
dimer | 2 * 54000, fetal liver enzyme, SDS-PAGE | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrobiopterin | - |
Homo sapiens | |
tetrahydrobiopterin | - |
Rattus norvegicus |