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Literature summary for 1.14.16.1 extracted from

  • Shiman, R.
    Purification and assay of rat liver phenylalanine 4-monooxygenase (1987), Methods Enzymol., 142, 17-27.
    View publication on PubMed
Search for more literature for 1.14.16.1

Activating Compound

Activating Compound Comment Organism Structure
lysolecithin
-
 Rattus norvegicus
additional information
-
 Rattus norvegicus
phenylalanine major regulator of liver enzyme, binds at an effector site converting the inactive to an active enzyme Rattus norvegicus
Phospholipids activate Rattus norvegicus

General Stability

General Stability Organism
frozen and thawed enzyme is 10-20% less active than before freezing Rattus norvegicus
glycerol + EDTA stabilize Rattus norvegicus
NaCl or KCl above 200 mM stabilize Rattus norvegicus
nonionic detergents e.g. Triton X-100 and Tween 80, 0.03-0.1%, stabilize Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
(6R)-L-erythro-tetrahydrobiopterin inhibits phenylalanine and lysolecithin activation Rattus norvegicus
catechol
-
 Rattus norvegicus
glycerol
-
 Rattus norvegicus
H2O2 inactivates the reduced form of the enzyme Rattus norvegicus
Tris
-
 Rattus norvegicus
Tween 80
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01 0.015 tetrahydrobiopterin substrate phenylalanine Rattus norvegicus
0.045
-
 6-methyltetrahydropterin substrate phenylalanine Rattus norvegicus
0.18
-
 phenylalanine cofactor 6-methyltetrahydropterin Rattus norvegicus
0.2
-
 phenylalanine cofactor tetrahydrobiopterin Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Rattus norvegicus 5737
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification
-
 Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.5 13
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-70°C, 2 years, no loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydrobiopterin + O2 additional electron donors: 6-methylpterin, 6,7-dimethyltetrahydropterin Rattus norvegicus L-tyrosine + dihydrobiopterin + H2O
-
 ?
L-phenylalanine + tetrahydrobiopterin + O2 additional electron donors: 6-methyltetrahydropterin, 7-methylpterin, and 2,4,5-triamino-6-hydroxypyrimidine Rattus norvegicus L-tyrosine + dihydrobiopterin + H2O
-
 ?
L-phenylalanine + tetrahydrobiopterin + O2 low activity with tetrahydrobiopterin can be selectively increased by a wide variety of reversible and irreversible modificators of the enzyme, e.g. interaction with phospholipids Rattus norvegicus L-tyrosine + dihydrobiopterin + H2O
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
additional information artificial cofactors: 6-methyltetrahydropterin, 6,7-dimethyltetrahydropterin Rattus norvegicus
additional information artificial cofactors: 6-methyltetrahydropterin, 7-methylpterin Rattus norvegicus
tetrahydrobiopterin
-
 Rattus norvegicus