Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cAMP-dependent protein kinase | - |
Homo sapiens | |
cAMP-dependent protein kinase | 2-4fold increase in activity in the presence of tetrahydrobiopterin | Rattus norvegicus | |
additional information | relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis | Homo sapiens | |
additional information | relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis | Rattus norvegicus | |
additional information | relatively low activity with tetrahydrobiopterin can be selectively increased by phosphorylation | Rattus norvegicus | |
additional information | non activated enzyme has much greater activity with 6-methyltetrahydropterin and dimethyltetrahydropterin than with tetrahydrobiopterin | Rattus norvegicus | |
N-ethylmaleimide | activation by alkylation of sulfhydryl groups | Rattus norvegicus | |
Phospholipids | activate | Homo sapiens | |
Phospholipids | activate | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | 110000 | - |
Rattus norvegicus |
200000 | 210000 | - |
Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | hepatic enzyme consists of a mixture of phosphorylated and nonphosphorylated forms | Rattus norvegicus |
side-chain modification | liver enzyme in untreated animals is a mixture of phosphorylated and nonphosphorylated forms, enzyme may be phosphorylated in vivo by cAMP-dependent protein kinase | Rattus norvegicus |
side-chain modification | activity of liver enzyme might be regulated by phosphorylation-dephosphorylation | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + tetrahydrobiopterin + O2 | relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis, alkylation of sulfhydryl groups with N-ethylmaleimide or phosphorylation by cAMP-dependent protein kinase | Rattus norvegicus | L-tyrosine + dihydrobiopterin + H2O | - |
? | |
L-phenylalanine + tetrahydrobiopterin + O2 | relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis | Homo sapiens | L-tyrosine + dihydrobiopterin + H2O | - |
? | |
L-phenylalanine + tetrahydrobiopterin + O2 | relatively low activity with tetrahydrobiopterin can be selectively increased by limited proteolysis | Rattus norvegicus | L-tyrosine + dihydrobiopterin + H2O | - |
? | |
L-phenylalanine + tetrahydrobiopterin + O2 | non activated enzyme has much greater activity with 6-methyltetrahydropterin and dimethyltetrahydropterin than with tetrahydrobiopterin | Rattus norvegicus | L-tyrosine + dihydrobiopterin + H2O | - |
? | |
L-phenylalanine + tetrahydrobiopterin + O2 | low activity with tetrahydrobiopterin can be selectively increased by a wide variety of reversible and irreversible modificators of the enzyme, e.g. interaction with phospholipids | Homo sapiens | L-tyrosine + dihydrobiopterin + H2O | - |
? | |
L-phenylalanine + tetrahydrobiopterin + O2 | low activity with tetrahydrobiopterin can be selectively increased by a wide variety of reversible and irreversible modificators of the enzyme, e.g. interaction with phospholipids | Rattus norvegicus | L-tyrosine + dihydrobiopterin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | - |
Homo sapiens |
More | some authors report that the enzyme exists as a mixture of dimers and tetramers, others report that it exists solely as tetramer or as dimer, percentage of dimers increases on frozen storage, preincubation of the enzyme with phenylalanine leads to even higher than 200000 Da molecular weight forms | Rattus norvegicus |
tetramer | tetrameric species accounts for 78% of the total enzyme | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrobiopterin | - |
Homo sapiens | |
tetrahydrobiopterin | - |
Rattus norvegicus |