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Literature summary for 1.14.15.7 extracted from

  • Brouquisse, R.; Weigel, P.; Rhodes, D.; Yocum, C.F.; Hanson, A.D.
    Evidence for a ferredoxin-dependent choline monooxygenase from spinach chloroplast stroma (1989), Plant Physiol., 90, 322-329.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
catalase stimulation of activity by countering negative effects of H2O2 produced by ferredoxin autoxidation Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
betaine 300 mM betaine give 50% inhibition Spinacia oleracea
Zn2+ 10 mM inhibits activity Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
choline reduced ferredoxin provided by NADPH/ferredoxin-NADP+ reductase or reconstituted chloroplasts Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast in stromal fraction of chloroplasts Spinacia oleracea 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10 mM Ca2+ can replace Mg2+ Spinacia oleracea
Mg2+ maximum activity with more than 10 mM Spinacia oleracea
additional information Mn2+, Cu2+ or Co2+ have no effect on activity Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
SDS-PAGE Spinacia oleracea
98000
-
gel filtration with Ultrogel AcA 34 Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
spinach, cv. Savoy Hybrid 612
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00025
-
activity varies with leaf age Spinacia oleracea
0.00058
-
after salinization of plants with 200 mM NaCl for two weeks Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
choline + O2 + reduced ferredoxin
-
Spinacia oleracea betaine aldehyde hydrate + oxidized ferredoxin
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
reduced ferredoxin provided by NADPH/ferredoxin-NADP+ reductase or reconstituted chloroplasts Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
Ferredoxin ferredoxin is reduced via NADPH and ferredoxin-NADP+ reductase Spinacia oleracea
O2 half-maximal rate with a O2 partial pressure of 2kPa, corresponding to an concentration of 0.025 mM Spinacia oleracea