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Literature summary for 1.14.15.6 extracted from

  • Warne, P.A.; Greenfield, N.J.; Lieberman, S.
    Modulation of the kinetics of cholesterol side-chain cleavage by an activator and by an inhibitor isolated from the cytosol of the cortex of bovine adrenals (1983), Proc. Natl. Acad. Sci. USA, 80, 1877-1881.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.15.6

Activating Compound

Activating Compound Comment Organism Structure
additional information activator of cholesterol side-chain cleavage isolated from the cytosol of bovine adrenal cortex Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
cholesterol inhibition above 0.003 mM, mitochondrial Bos taurus
Cholesterol sulfate inhibition above 0.005 mM, mitochondrial Bos taurus
additional information no substrate inhibition with cytosolic enzyme, inhibitor of cholesterol-side-chain cleavage isolated from the cytosol of bovine adrenal cortex Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
 Cholesterol sulfate mitochondrial Bos taurus
0.0005
-
 cholesterol mitochondrial Bos taurus
0.0165
-
 cholesterol cytosolic Bos taurus
0.0232
-
 Cholesterol sulfate cytosolic Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Bos taurus 5829
-
mitochondrial inner membrane inner membrane Bos taurus 5743
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + 6 reduced adrenodoxin + 3 O2 Bos taurus first step in biosynthesis of all steroid hormones pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O isocaproic aldehyde additional product ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
column chromatography, gel filtration Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal cortex
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + 6 reduced adrenodoxin + 3 O2 first step in biosynthesis of all steroid hormones Bos taurus pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O isocaproic aldehyde additional product ?
cholesterol + reduced adrenodoxin + O2
-
 Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 ?
cholesterol sulfate + reduced adrenodoxin + O2
-
 Bos taurus pregnenolone sulfate + 17-hydroxy-pregnenolone + dehydroisoandrosterone sulfate + oxidized adrenodoxin + H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
adrenodoxin
-
 Bos taurus
NADPH NADPH-linked adrenodoxin reductase Bos taurus