Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.15.6 extracted from

  • Dhariwal, M.S.; Jefcoate, C.R.
    Cholesterol metabolism by purified cytochrome P-450scc is highly stimulated by octyl glucoside and stearic acid exclusively in large unilamellar phospholipid vesicles (1989), Biochemistry, 28, 8397-8402.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cardiolipin affinity of P-450scc for cholesterol is increased Bos taurus
fatty acid C18, natural detergents in DMPC vesicles, stimulation similar to octyl glucoside Bos taurus
octyl glucoside high concentrations of this detergent cause 50% stimulation of cholesterol side-chain-cleavage in large unilamellar vesicles at low cholesterol concentration, 0.01 mM increase the proportion of P-450 bound by cholesterol Bos taurus
Phospholipid P-450scc reconstituted in vesicles, cholate-solubilized for small unilamellar dioleoylphosphatidylcholine vesicles, non-ionic detergent n-octyl glucoside used for large ones Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
cholesterol low concentration, both vesicle types Bos taurus
0.3
-
cholesterol large unilamellar vesicles, second type of reaction Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane matrix side Bos taurus 5743
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + 6 reduced adrenodoxin + 3 O2 Bos taurus first step in biosynthesis of all steroid hormones pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, affinity chromatography Bos taurus
DEAE-cellulose, adrenodoxin-Sepharose Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal cortex
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + 6 reduced adrenodoxin + 3 O2 first step in biosynthesis of all steroid hormones Bos taurus pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O
-
?
cholesterol + reduced adrenodoxin + O2
-
Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
?
additional information transfer of cholesterol to cytochrome P-450scc is rate-limiting Bos taurus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information for small unilamellar vesicles 5fold lower Bos taurus
0.417 0.5 cholesterol by large unilamellar vesicles Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
adrenodoxin required Bos taurus
NADPH
-
Bos taurus