Protein Variants | Comment | Organism |
---|---|---|
A245S | significantly increased activity with substrates testosterone and 7-benzyloxyquinoline. Contrary to wild-type, binding to inhibitor ketoconazole follows type II | Saccharopolyspora erythraea |
A245T | increased activity with substrate 7-benzyloxyquinoline. Like wild-type, type-II binding to inhibitor ketoconazole | Saccharopolyspora erythraea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ketoconazole | wild-type and mutant A245S show type-II binding. Mutant A245T shows type-I binding | Saccharopolyspora erythraea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0103 | - |
9-aminophenanthrene | wild-type, pH 7.5, 37°C, S50 value, Hill coefficient 1.9 | Saccharopolyspora erythraea | |
0.0312 | - |
9-aminophenanthrene | mutant A245T, pH 7.5, 37°C, S50 value, Hill coefficient 1.7 | Saccharopolyspora erythraea | |
0.261 | - |
androstenedione | wild-type, pH 7.5, 37°C, S50 value, Hill coefficient 1.2 | Saccharopolyspora erythraea | |
0.277 | - |
androstenedione | mutant A245T, pH 7.5, 37°C, S50 value, Hill coefficient 1.09 | Saccharopolyspora erythraea | |
0.278 | - |
testosterone | wild-type, pH 7.5, 37°C, S50 value, Hill coefficient 1.64 | Saccharopolyspora erythraea | |
0.326 | - |
dehydroepiandrostenedione | wild-type, pH 7.5, 37°C, S50 value, Hill coefficient 1.55 | Saccharopolyspora erythraea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharopolyspora erythraea | Q00441 | - |
- |
Saccharopolyspora erythraea NRRL 2338 | Q00441 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
9-aminophenanthrene + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Saccharopolyspora erythraea | ? + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
9-aminophenanthrene + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Saccharopolyspora erythraea NRRL 2338 | ? + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
androstenedione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Saccharopolyspora erythraea | ? + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
androstenedione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Saccharopolyspora erythraea NRRL 2338 | ? + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
dehydroepiandrostenedione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Saccharopolyspora erythraea | ? + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
dehydroepiandrostenedione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Saccharopolyspora erythraea NRRL 2338 | ? + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
additional information | enzyme demonstrates cooperative ligand binding and oxidation kinetics | Saccharopolyspora erythraea | ? | - |
? | |
additional information | enzyme demonstrates cooperative ligand binding and oxidation kinetics | Saccharopolyspora erythraea NRRL 2338 | ? | - |
? | |
testosterone + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Saccharopolyspora erythraea | ? + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
testosterone + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Saccharopolyspora erythraea NRRL 2338 | ? + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytochrome P450eryF | - |
Saccharopolyspora erythraea |
eryF | - |
Saccharopolyspora erythraea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Saccharopolyspora erythraea |