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Literature summary for 1.14.15.30 extracted from

  • Yao, K.; Xu, L.Q.; Wang, F.Q.; Wei, D.Z.
    Characterization and engineering of 3-ketosteroid-Delta1-dehydrogenase and 3-ketosteroid-9alpha-hydroxylase in Mycobacterium neoaurum ATCC 25795 to produce 9alpha-hydroxy-4-androstene-3,17-dione through the catabolism of sterols (2014), Metab. Eng., 24, 181-191 .
    View publication on PubMed
Search for more literature for 1.14.15.30

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycolicibacterium neoaurum

Protein Variants

Protein Variants Comment Organism
additional information recombinant coexpression of the enzyme KshA from Mycobacterium neoaurum strain NwIB-01 and the three homologues of ketosteroid-DELTA1-dehydrogenase (KstD) from Mycobacterium neoaurum ATCC257953 in Escherichia coli strain BL21(DE3) Mycolicibacterium neoaurum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a Rieske 2Fe-2S enzyme Mycolicibacterium neoaurum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 Mycolicibacterium neoaurum
-
 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 Mycolicibacterium neoaurum NwIB-01
-
 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 Mycolicibacterium neoaurum ATCC 25795
-
 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium neoaurum C9E9N6
-
-
Mycolicibacterium neoaurum ATCC 25795 C9E9N6
-
-
Mycolicibacterium neoaurum NwIB-01 C9E9N6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Mycolicibacterium neoaurum 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Mycolicibacterium neoaurum NwIB-01 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Mycolicibacterium neoaurum ATCC 25795 9alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

Synonyms Comment Organism
3-ketosteroid-9alpha-hydroxylase
-
 Mycolicibacterium neoaurum
KSH
-
 Mycolicibacterium neoaurum
KshA
-
 Mycolicibacterium neoaurum
KshA oxygenase component of 3-ketosteroid-9alpha-hydroxylase Mycolicibacterium neoaurum
MN-kshA
-
 Mycolicibacterium neoaurum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mycolicibacterium neoaurum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Mycolicibacterium neoaurum

Cofactor

Cofactor Comment Organism Structure
[2Fe-2S]-center a Rieske 2Fe-2S enzyme Mycolicibacterium neoaurum

Expression

Organism Comment Expression
Mycolicibacterium neoaurum recombinant coexpression of the enzyme KshA from Mycobacterium neoaurum strain NwIB-01 and the three homologues of ketosteroid-DELTA1-dehydrogenase (KstD) from Mycobacterium neoaurum ATCC257953 in Escherichia coli strain BL21(DE3),to install a steroid production system with a broad substrate specificity. Enzyme KshA converts the KstD reaction side product 4-androstene-3,17-dione into 9alpha-hydroxyandrosta-1,4-diene-3,17-dione additional information