Literature summary for 1.14.15.3 extracted from

Alonso, H.; Kleifeld, O.; Yeheskel, A.; Ong, P.C.; Liu, Y.C.; Stok, J.E.; De Voss, J.J.; Roujeinikova, A.
Structural and mechanistic insight into alkane hydroxylation by Pseudomonas putida AlkB (2014), Biochem. J., 460, 283-293 .

Search for more literature for 1.14.15.3

Crystallization (Commentary)

Crystallization (Comment)	Organism
molecular modeling of the transmembrane domain	Pseudomonas putida

Protein Variants

Protein Variants	Comment	Organism
K18A	complete loss of activity, mutant protein display an altered secondary structure and decreased melting temperature	Pseudomonas putida
K193A	5-20% loss of activity	Pseudomonas putida
K359A	5-20% loss of activity	Pseudomonas putida

Inhibitors

Inhibitors	Comment	Organism	Structure
1-octyne	irreversible inhibition, oxidation product hexylketene or its equivalent, covalently binds to AlkB	Pseudomonas putida

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Pseudomonas putida	16020	-

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 1-octyne + 2 NADH + 2 H+ + 2 O2	-	Pseudomonas putida	1-octanoic acid + 7-octynoic acid + 2 NAD+ + 2 H2O	-	?
n-octane + NADH + H+ + O2	-	Pseudomonas putida	n-octanol + NAD+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
AlkB	-	Pseudomonas putida
Gpo1	-	Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
71.2	-	melting temperature, mutant K18A	Pseudomonas putida
74.5	-	melting temperature, wild-type	Pseudomonas putida
75.3	-	melting temperature, mutant K359A	Pseudomonas putida
78.7	-	melting temperature, mutant K193A	Pseudomonas putida

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Release 2023.1 (January 2023)