Cloned (Comment) | Organism |
---|---|
recombinant expression of His6-tagged enzyme system components in Escherichia coli strain BL21(DE3) | Pusillimonas sp. T7-7 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Pusillimonas sp. T7-7 | |
Co2+ | - |
Pusillimonas sp. T7-7 | |
Cu2+ | - |
Pusillimonas sp. T7-7 | |
EDTA | - |
Pusillimonas sp. T7-7 | |
Mg2+ | - |
Pusillimonas sp. T7-7 | |
Mn2+ | - |
Pusillimonas sp. T7-7 | |
Ni2+ | - |
Pusillimonas sp. T7-7 | |
Pb2+ | - |
Pusillimonas sp. T7-7 | |
Zn2+ | - |
Pusillimonas sp. T7-7 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0182 | - |
pentadecane | pH 7.5, 30°C, recombinant alkane hydroxylase large subunit | Pusillimonas sp. T7-7 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Al3+ | activates | Pusillimonas sp. T7-7 | |
Fe2+ | required for the ferredoxin cofactor, activates | Pusillimonas sp. T7-7 | |
additional information | no or poor effects by Na and K+ | Pusillimonas sp. T7-7 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
3 * 53000, recombinant His6-tagged alkane hydroxylase large subunit, SDS-PAGE | Pusillimonas sp. T7-7 |
160000 | - |
recombinant His6-tagged alkane hydroxylase large subunit, native PAGE | Pusillimonas sp. T7-7 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pusillimonas sp. T7-7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme system components from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pusillimonas sp. T7-7 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | strain T7-7 is able to utilize diesel oils, C5 to C30 alkane, as a sole carbon and energy source | Pusillimonas sp. T7-7 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-hexadecene + reduced rubredoxin + O2 | - |
Pusillimonas sp. T7-7 | ? + oxidized rubredoxin + H2O | - |
? | |
1-octadecene + reduced rubredoxin + O2 | - |
Pusillimonas sp. T7-7 | ? + oxidized rubredoxin + H2O | - |
? | |
decane + reduced rubredoxin + O2 | - |
Pusillimonas sp. T7-7 | 1-decanol + oxidized rubredoxin + H2O | - |
? | |
dodecane + reduced rubredoxin + O2 | - |
Pusillimonas sp. T7-7 | 1-dodecanol + oxidized rubredoxin + H2O | - |
? | |
heptadecane + reduced rubredoxin + O2 | - |
Pusillimonas sp. T7-7 | 1-heptadecanol + oxidized rubredoxin + H2O | - |
? | |
hexadecane + reduced rubredoxin + O2 | - |
Pusillimonas sp. T7-7 | 1-hexadecanol + oxidized rubredoxin + H2O | - |
? | |
hexane + reduced rubredoxin + O2 | - |
Pusillimonas sp. T7-7 | 1-hexanol + oxidized rubredoxin + H2O | - |
? | |
methane sulfonic acid + reduced rubredoxin + O2 | - |
Pusillimonas sp. T7-7 | ? + oxidized rubredoxin + H2O | - |
? | |
additional information | substrate specificity of the alkane hydroxylase large subunit, overview. Poor activity with 1-tetradecanol, no activity with octacosane, benzene, sodium benzoate, toluene, catechol, biphenyl, 1-hexadecanol, 1-stearyl alcohol, isooctane, pristine, and squalane | Pusillimonas sp. T7-7 | ? | - |
? | |
nitromethane + reduced rubredoxin + O2 | - |
Pusillimonas sp. T7-7 | ? + oxidized rubredoxin + H2O | - |
? | |
octadecane + reduced rubredoxin + O2 | - |
Pusillimonas sp. T7-7 | 1-octadecanol + oxidized rubredoxin + H2O | - |
? | |
octane + reduced rubredoxin + O2 | - |
Pusillimonas sp. T7-7 | 1-octanol + oxidized rubredoxin + H2O | - |
? | |
pentadecane + reduced rubredoxin + O2 | best substrate | Pusillimonas sp. T7-7 | 1-pentadecanol + oxidized rubredoxin + H2O | - |
? | |
pentane + reduced rubredoxin + O2 | - |
Pusillimonas sp. T7-7 | 1-pentanol + oxidized rubredoxin + H2O | - |
? | |
tetracosane + reduced rubredoxin + O2 | - |
Pusillimonas sp. T7-7 | 1-tetracosanol + oxidized rubredoxin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the alkane hydroxylase system consists of the alkane hydroxylase large subunit, the alkane hydroxylase small subunit, the NADH-dependent reductase subunit, and the ferredoxin subunit | Pusillimonas sp. T7-7 |
trimer | 3 * 53000, recombinant His6-tagged alkane hydroxylase large subunit, SDS-PAGE | Pusillimonas sp. T7-7 |
Synonyms | Comment | Organism |
---|---|---|
alkane hydroxylase | - |
Pusillimonas sp. T7-7 |
Rieske-type alkane monooxygenase | - |
Pusillimonas sp. T7-7 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
alkane hydroxylase large subunit | Pusillimonas sp. T7-7 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
alkane hydroxylase large subunit | Pusillimonas sp. T7-7 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | Rieske-type alkane monooxygenase with Rieske-type [2Fe-2S]2 center | Pusillimonas sp. T7-7 | |
NADH | NADH-dependent alkane monooxygenase activity (C5 to C24), the alkane oxidation is coupled to NADH consumption | Pusillimonas sp. T7-7 |
General Information | Comment | Organism |
---|---|---|
additional information | the alkane hydroxylase system consists of the alkane hydroxylase large subunit, the alkane hydroxylase small subunit, the NADH-dependent reductase subunit, and the ferredoxin subunit. Analysis of the interaction between large and small subunits of the monooxygenase, overview | Pusillimonas sp. T7-7 |