Application | Comment | Organism |
---|---|---|
synthesis | bacterial alkane hydroxylases have tremendous potential as biocatalysts for the stereo- and regioselective transformation of a wide range of chemically inert unreactive alkanes into valuable reactive chemical precursors | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
gene alkB, expression of Strep-tagged enzyme in Escherichia coli strains BL21(DE3), C41(DE3), Rosetta-2(DE3), pLysS, and BL21-CondonPlus(DE3)-RIPL under different growth conditions | Pseudomonas putida |
Crystallization (Comment) | Organism |
---|---|
2D crystallization | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-octyne | acts as a mechanism-based inhibitor of AlkB | Pseudomonas putida | |
n-decyl-beta-D-maltopyranoside | - |
Pseudomonas putida | |
n-dodecyl-beta-D-maltopyranoside | - |
Pseudomonas putida | |
n-dodecyl-N,N-dimethylamine-n-oxide | - |
Pseudomonas putida | |
octyl glucose neopentyl glycol | - |
Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | AlkB is the integral membrane component of the Pseudomonas putida alkane hydroxylase system | Pseudomonas putida | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, recombinant Strep-tagged AlkB, SDS-PAGE | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
octane + reduced rubredoxin + O2 | Pseudomonas putida | - |
1-octanol + oxidized rubredoxin + H2O | - |
? | |
octane + reduced rubredoxin + O2 | Pseudomonas putida GPo1 / ATCC29347 | - |
1-octanol + oxidized rubredoxin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida GPo1 / ATCC29347 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant Strep-tagged enzyme from Escherichia coli strains BL21(DE3), C41(DE3), Rosetta-2(DE3), pLysS, and BL21-CondonPlus(DE3)-RIPL by Strep-tag affinity chromatography, ultrafiltration, and gel filtration | Pseudomonas putida |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the alkane hydroxylase system of Pseudomonas putida GPo1 allows it to use alkanes as the sole source of carbon and energy | Pseudomonas putida | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
octane + reduced rubredoxin + O2 | - |
Pseudomonas putida | 1-octanol + oxidized rubredoxin + H2O | - |
? | |
octane + reduced rubredoxin + O2 | - |
Pseudomonas putida GPo1 / ATCC29347 | 1-octanol + oxidized rubredoxin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, recombinant Strep-tagged AlkB, SDS-PAGE | Pseudomonas putida |
More | the physiologically active form of membrane-embedded AlkB may be a multimer, the monomeric form of AlkB produced by purification in n-decyl-beta-D-maltopyranoside, n-dodecyl-beta-D-maltopyranoside, octyl glucose neopentyl glycol, and n-dodecyl-N,N-dimethylamine-N-oxide is largely inactive. In contrast, detergents that do not disrupt the AlkB oligomeric state, i.e. decyl maltose neopentyl glycol, lauryl maltose neopentyl glycol, and octaethylene glycol monododecyl ether, preserve the enzyme activity at a level close to that of the detergent-free control sample | Pseudomonas putida |
trimer | AlkB reconstituted into a lipid bilayer forms trimers | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
AlkB | - |
Pseudomonas putida |
medium-chain alkane hydroxylase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
purified recombinant enzyme, 15 min, inactivation | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
rubredoxin | - |
Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
physiological function | the alkane hydroxylase system of Pseudomonas putida GPo1 allows it to use alkanes as the sole source of carbon and energy. The alkane hydroxylase system of Pseudomonas putida GPo1 comprises three protein components: AlkB, soluble NADH-rubredoxin reductase, and soluble electron transfer protein rubredoxin. AlkB transfers one oxygen atom from O2 to the alkane molecule, while the other oxygen is reduced to H2O using the electrons provided by NADH-rubredoxin reductase via rubredoxin | Pseudomonas putida |