Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS | Pseudomonas oleovorans |
Protein Variants | Comment | Organism |
---|---|---|
H138A | no activity | Pseudomonas oleovorans |
H142A | no activity | Pseudomonas oleovorans |
H163A | ~50% of activity | Pseudomonas oleovorans |
H168A | no activity | Pseudomonas oleovorans |
H172A | no activity | Pseudomonas oleovorans |
H173A | no activity | Pseudomonas oleovorans |
H181A | no activity | Pseudomonas oleovorans |
H308A | ~30% of activity | Pseudomonas oleovorans |
H312A | no activity | Pseudomonas oleovorans |
H315A | no activity | Pseudomonas oleovorans |
H316A | no activity | Pseudomonas oleovorans |
H318A | ~25% of activity | Pseudomonas oleovorans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Pseudomonas oleovorans | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | required, diiron enzyme | Pseudomonas oleovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
n-alkane + NADPH + H+ + O2 | Pseudomonas oleovorans | initial activation of alkanes | n-alkanol + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas oleovorans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pseudomonas oleovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.57 | - |
H318A mutant enzyme, 22°C | Pseudomonas oleovorans |
0.67 | - |
H308A mutant enzyme, 22°C | Pseudomonas oleovorans |
1.11 | - |
H163A mutant enzyme, 22°C | Pseudomonas oleovorans |
2.03 | - |
native enzyme, 22°C | Pseudomonas oleovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
n-alkane + NADPH + H+ + O2 | - |
Pseudomonas oleovorans | n-alkanol + NADP+ + H2O | - |
? | |
n-alkane + NADPH + H+ + O2 | initial activation of alkanes | Pseudomonas oleovorans | n-alkanol + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alkane omega-hydroxylase | - |
Pseudomonas oleovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Pseudomonas oleovorans |