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Literature summary for 1.14.15.3 extracted from

  • Shanklin, J.; Whittle, E.
    Evidence linking the Pseudomonas oleovorans alkane omega-hydroxylase, an integral membrane diiron enzyme, and the fatty acid desaturase family (2003), FEBS Lett., 545, 188-192.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS Pseudomonas oleovorans

Protein Variants

Protein Variants Comment Organism
H138A no activity Pseudomonas oleovorans
H142A no activity Pseudomonas oleovorans
H163A ~50% of activity Pseudomonas oleovorans
H168A no activity Pseudomonas oleovorans
H172A no activity Pseudomonas oleovorans
H173A no activity Pseudomonas oleovorans
H181A no activity Pseudomonas oleovorans
H308A ~30% of activity Pseudomonas oleovorans
H312A no activity Pseudomonas oleovorans
H315A no activity Pseudomonas oleovorans
H316A no activity Pseudomonas oleovorans
H318A ~25% of activity Pseudomonas oleovorans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Pseudomonas oleovorans 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron required, diiron enzyme Pseudomonas oleovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
n-alkane + NADPH + H+ + O2 Pseudomonas oleovorans initial activation of alkanes n-alkanol + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas oleovorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.57
-
H318A mutant enzyme, 22°C Pseudomonas oleovorans
0.67
-
H308A mutant enzyme, 22°C Pseudomonas oleovorans
1.11
-
H163A mutant enzyme, 22°C Pseudomonas oleovorans
2.03
-
native enzyme, 22°C Pseudomonas oleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
n-alkane + NADPH + H+ + O2
-
Pseudomonas oleovorans n-alkanol + NADP+ + H2O
-
?
n-alkane + NADPH + H+ + O2 initial activation of alkanes Pseudomonas oleovorans n-alkanol + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
alkane omega-hydroxylase
-
Pseudomonas oleovorans

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Pseudomonas oleovorans