Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain XL1 and SCS1 | Oryctolagus cuniculus |
Protein Variants | Comment | Organism |
---|---|---|
H206Y | gene CYP4A7 | Oryctolagus cuniculus |
H206Y/S255F | gene CYP4A7 | Oryctolagus cuniculus |
R90W | gene CYP4A7 | Oryctolagus cuniculus |
R90W/W93S | gene CYP4A7 | Oryctolagus cuniculus |
S255F | gene CYP4A7 | Oryctolagus cuniculus |
W93S | gene CYP4A7 | Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | - |
Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonic acid + NADPH + H+ + O2 | Oryctolagus cuniculus | - |
20-hydroxyeicosatetraenoic acid + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
rabbit | - |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonic acid + NAD(P)H + H+ + O2 | - |
Oryctolagus cuniculus | 20-hydroxyicosa-5,8,11,14-tetraenoic acid + NAD(P)+ + H2O | - |
? | |
arachidonic acid + NADPH + H+ + O2 | - |
Oryctolagus cuniculus | 20-hydroxyeicosatetraenoic acid + NADP+ + H2O | - |
? | |
fatty acid + NAD(P)H + H+ + O2 | laurate and arachidonic acid | Oryctolagus cuniculus | omega-hydroxy fatty acid + NAD(P)+ + H2O | - |
? | |
fatty acid + NAD(P)H + H+ + O2 | NADPH-P450 reductase and cytochrome b5 are part of the enzyme system | Oryctolagus cuniculus | omega-hydroxy fatty acid + NAD(P)+ + H2O | - |
? | |
lauric acid + NAD(P)H + H+ + O2 | - |
Oryctolagus cuniculus | 12-hydroxydodecanoic acid + NAD(P)+ + H2O | - |
? | |
prostaglandin E1 + NADPH + O2 | - |
Oryctolagus cuniculus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0267 | - |
laurate | CYP4A7, R90W mutant, omega-hydroxylation | Oryctolagus cuniculus | |
0.117 | - |
laurate | CYP4A4wt, omega-hydroxylation | Oryctolagus cuniculus | |
0.167 | - |
arachidonic acid | CYP4A7, R90W mutant, omega-hydroxylation | Oryctolagus cuniculus | |
0.183 | - |
prostaglandin E1 | CYP4A7wt, omega-hydroxylation | Oryctolagus cuniculus | |
0.2 | - |
laurate | CYP4A7, R90W/W93S mutant, omega-hydroxylation | Oryctolagus cuniculus | |
0.217 | - |
arachidonic acid | CYP4A7, R90W/W93S mutant, omega-hydroxylation | Oryctolagus cuniculus | |
0.25 | - |
prostaglandin E1 | CYP4A7, R90W/W93S mutant, omega-hydroxylation | Oryctolagus cuniculus | |
0.283 | - |
prostaglandin E1 | CYP4A7, R90W mutant, omega-hydroxylation | Oryctolagus cuniculus | |
0.3 | - |
prostaglandin E1 | CYP4A7, W93S mutant, omega-hydroxylation | Oryctolagus cuniculus | |
0.3 | - |
prostaglandin E1 | CYP4A7, S255F mutant, omega-hydroxylation | Oryctolagus cuniculus | |
0.3 | - |
prostaglandin E1 | CYP4A7, H206Y/S255F mutant, omega-hydroxylation | Oryctolagus cuniculus | |
0.383 | - |
prostaglandin E1 | CYP4A4wt, omega-hydroxylation | Oryctolagus cuniculus | |
0.417 | - |
prostaglandin E1 | CYP4A7, H206Y mutant, omega-hydroxylation | Oryctolagus cuniculus | |
0.45 | - |
arachidonic acid | CYP4A7, W93S mutant, omega-hydroxylation | Oryctolagus cuniculus | |
0.5 | - |
laurate | CYP4A7, W93S mutant, omega-hydroxylation | Oryctolagus cuniculus | |
0.717 | - |
arachidonic acid | CYP4A7wt, omega-hydroxylation | Oryctolagus cuniculus | |
0.767 | - |
laurate | CYP4A7wt, omega-hydroxylation | Oryctolagus cuniculus | |
0.783 | - |
arachidonic acid | CYP4A4wt, omega-hydroxylation | Oryctolagus cuniculus | |
2.15 | - |
arachidonic acid | CYP4A7, S255F mutant, omega-hydroxylation | Oryctolagus cuniculus | |
2.17 | - |
arachidonic acid | CYP4A7, H206Y/S255F mutant, omega-hydroxylation | Oryctolagus cuniculus | |
2.23 | - |
laurate | CYP4A7, H206Y mutant, omega-hydroxylation | Oryctolagus cuniculus | |
2.33 | - |
arachidonic acid | CYP4A7, H206Y mutant, omega-hydroxylation | Oryctolagus cuniculus | |
2.45 | - |
laurate | CYP4A7, H206Y/S255F mutant, omega-hydroxylation | Oryctolagus cuniculus | |
2.67 | - |
laurate | CYP4A7, S255F mutant, omega-hydroxylation | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Oryctolagus cuniculus |