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Literature summary for 1.14.15.3 extracted from

  • Nakayama, N.; Takemae, A.; Shoun, H.
    Cytochrome P450foxy, a catalytically self-sufficient fatty acid hydroxylase of the fungus Fusarium oxysporum (1996), J. Biochem., 119, 435-440.
    View publication on PubMed
Search for more literature for 1.14.15.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 lauric acid
-
 Fusarium oxysporum

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Fusarium oxysporum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
118000
-
 SDS-PAGE Fusarium oxysporum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
n-alkane + NAD(P)H + H+ + O2 Fusarium oxysporum
-
 n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2 Fusarium oxysporum MT-811
-
 n-alkanol + NAD(P)+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum
-
-
-
Fusarium oxysporum MT-811
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Fusarium oxysporum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.5
-
 hydroxylation of lauric acid Fusarium oxysporum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(n-1)-alkanoate + NADPH + H+ + O2
-
 Fusarium oxysporum (omega-1)-hydroxy-n-alkanoate + NADP+ + H2O
-
 ?
(n-1)-alkanoate + NADPH + H+ + O2
-
 Fusarium oxysporum MT-811 (omega-1)-hydroxy-n-alkanoate + NADP+ + H2O
-
 ?
fatty acid + NAD(P)H + H+ + O2
-
 Fusarium oxysporum omega-hydroxy fatty acid + NAD(P)+ + H2O
-
 ?
fatty acid + NAD(P)H + H+ + O2
-
 Fusarium oxysporum MT-811 omega-hydroxy fatty acid + NAD(P)+ + H2O
-
 ?
lauric acid + NAD(P)H + H+ + O2
-
 Fusarium oxysporum 12-hydroxydodecanoic acid + NAD(P)+ + H2O
-
 ?
lauric acid + NAD(P)H + H+ + O2
-
 Fusarium oxysporum MT-811 12-hydroxydodecanoic acid + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2
-
 Fusarium oxysporum n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2
-
 Fusarium oxysporum MT-811 n-alkanol + NAD(P)+ + H2O
-
 ?
palmitic acid + NAD(P)H + H+ + O2
-
 Fusarium oxysporum 16-hydroxyhexadecanoic acid + NAD(P)+ + H2O
-
 ?
palmitic acid + NAD(P)H + H+ + O2
-
 Fusarium oxysporum MT-811 16-hydroxyhexadecanoic acid + NAD(P)+ + H2O
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay conditions Fusarium oxysporum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay conditions Fusarium oxysporum

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Fusarium oxysporum
FMN
-
 Fusarium oxysporum
NADPH
-
 Fusarium oxysporum