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Literature summary for 1.14.15.3 extracted from

  • Scheller, U.; Zimmer, T.; Kaergel, E.; Schunck, W.H.
    Characterization of the n-alkane and fatty acid hydroxylating cytochrome P450 forms 52A3 and 52A4 (1996), Arch. Biochem. Biophys., 328, 245-254.
    View publication on PubMed
Search for more literature for 1.14.15.3

Activating Compound

Activating Compound Comment Organism Structure
Emulgen 911 needs detergent 0.3% Candida maltosa

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain GRF18 Candida maltosa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome integral membrane protein Candida maltosa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe
-
 Candida maltosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 gene expression CYP52A3, SDS-PAGE Candida maltosa
56500
-
 gene expression CYP52A4, SDS-PAGE Candida maltosa
59800
-
 gene expression CYP52A3, amino acid composition Candida maltosa
61800
-
 gene expression CYP52A4, amino acid composition Candida maltosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
n-alkane + NAD(P)H + H+ + O2 Candida maltosa
-
 n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2 Candida maltosa EH15
-
 n-alkanol + NAD(P)+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Candida maltosa
-
-
-
Candida maltosa EH15
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Candida maltosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fatty acid + NAD(P)H + H+ + O2 protein from CYP52A3 has a higher affinity for fatty acids than for alkanes Candida maltosa omega-hydroxy fatty acid + NAD(P)+ + H2O
-
 ?
fatty acid + NAD(P)H + H+ + O2 protein from CYP52A3 has a higher affinity for fatty acids than for alkanes Candida maltosa EH15 omega-hydroxy fatty acid + NAD(P)+ + H2O
-
 ?
lauric acid + NAD(P)H + H+ + O2
-
 Candida maltosa 12-hydroxydodecanoic acid + NAD(P)+ + H2O
-
 ?
lauric acid + NAD(P)H + H+ + O2
-
 Candida maltosa EH15 12-hydroxydodecanoic acid + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2
-
 Candida maltosa n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2 protein from CYP52A3 has a higher affinity for alkanes than for fatty acids Candida maltosa n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2
-
 Candida maltosa EH15 n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2 protein from CYP52A3 has a higher affinity for alkanes than for fatty acids Candida maltosa EH15 n-alkanol + NAD(P)+ + H2O
-
 ?
palmitic acid + NAD(P)H + H+ + O2
-
 Candida maltosa 16-hydroxyhexadecanoic acid + NAD(P)+ + H2O
-
 ?
palmitic acid + NAD(P)H + H+ + O2
-
 Candida maltosa EH15 16-hydroxyhexadecanoic acid + NAD(P)+ + H2O
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay conditions Candida maltosa

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Candida maltosa