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Literature summary for 1.14.15.3 extracted from

  • Ruettinger, R.T.; Griffith, G.R.; Coon, M.J.
    Characterization of the omega-hydroxylase of Pseudomonas oleovorans as a nonheme iron protein (1977), Arch. Biochem. Biophys., 183, 528-537.
    View publication on PubMed
Search for more literature for 1.14.15.3

General Stability

General Stability Organism
after 24 h 15 mM EDTA 60% activity and iron retained Pseudomonas oleovorans
after 4 h 15 mM EDTA and 10 mM dithionite 10% activity and iron retained Pseudomonas oleovorans

Inhibitors

Inhibitors Comment Organism Structure
KCN reversible inhibitor Pseudomonas oleovorans

Localization

Localization Comment Organism GeneOntology No. Textmining
cell membrane membrane-bound enzyme, needs 20 molecules phospholipid per polypeptide chain Pseudomonas oleovorans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu 0.03 atoms per 42000 g/mol peptide Pseudomonas oleovorans
Fe 1 atom Fe per 42000 g/mol peptide Pseudomonas oleovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40100
-
 x * 40100, amino acid composition Pseudomonas oleovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
n-alkane + NAD(P)H + H+ + O2 Pseudomonas oleovorans
-
 n-alkanol + NAD(P)+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas oleovorans
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 5% carbohydrate Pseudomonas oleovorans

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas oleovorans

Renatured (Commentary)

Renatured (Comment) Organism
ferrous ions restore activity after treatment with EDTA and dithionite Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.23
-
 hydroxylation of octane Pseudomonas oleovorans
1.75
-
 epoxidation of 1,7-octadiene Pseudomonas oleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,7-octadiene + NADH + H+ + O2 epoxidation of simple, aliphatic terminal olefins Pseudomonas oleovorans 1,2-epoxy-7-octene + NAD+ + H2O
-
 ?
fatty acid + NAD(P)H + H+ + O2
-
 Pseudomonas oleovorans omega-hydroxy fatty acid + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2
-
 Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
 ?
n-alkane + NAD(P)H + H+ + O2 rubredoxin is electron carrier Pseudomonas oleovorans n-alkanol + NAD(P)+ + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 40100, amino acid composition Pseudomonas oleovorans

Cofactor

Cofactor Comment Organism Structure
NADH hydroxylation needs also a NADH-dependent bacterial reductase Pseudomonas oleovorans