Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Phospholipid | enhances enzyme activity | Pseudomonas oleovorans |
General Stability | Organism |
---|---|
treatment with 0.1 M EDTA and 0.1 M dithionite reduces activity to 10% | Pseudomonas oleovorans |
treatment with sodium cholate and subsequent precipitation with ammonium sulfate reduces activity to 40% | Pseudomonas oleovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | non-heme iron protein | Pseudomonas oleovorans | |
Fe | 1 atom Fe per 42000 g/mol peptide | Pseudomonas oleovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE | Pseudomonas oleovorans |
2000000 | - |
- |
Pseudomonas oleovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
n-alkane + NAD(P)H + H+ + O2 | Pseudomonas oleovorans | - |
n-alkanol + NAD(P)+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas oleovorans | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
addition of dilauroylglyceryl-3-phosphorylcholine restores initial activity after treatment with sodium cholate and subsequent precipitation with ammonium sulfate | Pseudomonas oleovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.59 | - |
- |
Pseudomonas oleovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fatty acid + NAD(P)H + H+ + O2 | - |
Pseudomonas oleovorans | omega-hydroxy fatty acid + NAD(P)+ + H2O | - |
? | |
n-alkane + NAD(P)H + H+ + O2 | - |
Pseudomonas oleovorans | n-alkanol + NAD(P)+ + H2O | - |
? | |
n-alkane + NAD(P)H + H+ + O2 | rubredoxin is electron carrier | Pseudomonas oleovorans | n-alkanol + NAD(P)+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Pseudomonas oleovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay conditions | Pseudomonas oleovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | hydroxylation needs also a NADH-dependent bacterial reductase | Pseudomonas oleovorans |