Crystallization (Comment) | Organism |
---|---|
in complex with cholesteryl sulfate, sitting drop vapor diffusion method, using 20-25% PEG 3350, 10-13% (v/v) glycerol, and 50 mM MgCl2 | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0398 | - |
cholest-4-en-3-one | at pH 7.4 and 25°C | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin | Mycolicibacterium smegmatis | - |
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | A0R4Q6 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel-nitrilotriacetic acid column chromatography and Q-Sepharose column chromatography | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin | - |
Mycolicibacterium smegmatis | (25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP142A2 | - |
Mycolicibacterium smegmatis |