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Literature summary for 1.14.15.28 extracted from

  • Garcia-Fernandez, E.; Frank, D.J.; Galan, B.; Kells, P.M.; Podust, L.M.; Garcia, J.L.; Ortiz de Montellano, P.R.
    A highly conserved mycobacterial cholesterol catabolic pathway (2013), Environ. Microbiol., 15, 2342-2359.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.15.28

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0103
-
 cholest-4-en-3-one at pH 7.5 and 37°C Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin Mycolicibacterium smegmatis
-
 (25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O
-
 ?
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin Mycolicibacterium smegmatis mc(2)155
-
 (25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-
Mycolicibacterium smegmatis mc(2)155
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, SP-Sepharose column chromatography, and Q-Sepharose column chromatography Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin
-
 Mycolicibacterium smegmatis (25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O
-
 ?
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin
-
 Mycolicibacterium smegmatis mc(2)155 (25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP142A1 isoform Mycolicibacterium smegmatis
CYP142A2 isoform Mycolicibacterium smegmatis

Cofactor

Cofactor Comment Organism Structure
heme
-
 Mycolicibacterium smegmatis