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Literature summary for 1.14.15.24 extracted from

  • Wu, J.; Ji, J.; Wang, G.; Li, Z.; Diao, J.; Wu, G.
    Cloning and characterization of a novel beta-carotene hydroxylase gene from Lycium barbarum and its expression in Escherichia coli (2014), Biotechnol. Appl. Biochem., 61, 637-645 .
    View publication on PubMed
Search for more literature for 1.14.15.24

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Lycium barbarum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Lycium barbarum 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 Lycium barbarum
-
 zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Lycium barbarum U5Y6R4
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Lycium barbarum

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Lycium barbarum
-
fruit highest expression Lycium barbarum
-
leaf
-
 Lycium barbarum
-
root
-
 Lycium barbarum
-
stem lowest expression Lycium barbarum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Lycium barbarum zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Lycium barbarum
? x * 34800, calculated from amino acid sequence Lycium barbarum

Synonyms

Synonyms Comment Organism
beta-carotene hydroxylase
-
 Lycium barbarum
Chyb
-
 Lycium barbarum

pI Value

Organism Comment pI Value Maximum pI Value
Lycium barbarum calculated from amino acid sequence
-
 8.4