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Literature summary for 1.14.15.24 extracted from

  • Lu, Q.; Bu, Y.F.; Liu, J.Z.
    Metabolic engineering of Escherichia coli for producing astaxanthin as the predominant carotenoid (2017), Mar. Drugs, 15, E296 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis synthesis of astaxanthin. Astaxanthin is a carotenoid of significant commercial value due to its superior antioxidant potential and wide applications in the aquaculture, food, cosmetic and pharmaceutical industries. The Brevundimonas sp. SD212 crtW and Pantoea ananatis crtZ genes are the best combination for astaxanthin production. After balancing the activities of beta-carotene ketolase and hydroxylase, an Escherichia coli ASTA-1 that carries neither a plasmid nor an antibiotic marker is constructed to produce astaxanthin as the predominant carotenoid (96.6%) with a specific content of 7.4 mg/g dry cell weight without an addition of inducer Pantoea ananatis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pantoea ananatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2 Pantoea ananatis the enzyme plays an important role in astaxanthin production zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Pantoea ananatis D4GFL0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
Pantoea ananatis zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
?
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2 the enzyme plays an important role in astaxanthin production Pantoea ananatis zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
?

Synonyms

Synonyms Comment Organism
beta-carotene hydroxylase
-
Pantoea ananatis
CrtZ
-
Pantoea ananatis

General Information

General Information Comment Organism
metabolism the enzyme plays an important role in astaxanthin production Pantoea ananatis