Literature summary for 1.14.15.24 extracted from

Wang, R.; Gu, X.; Yao, M.; Pan, C.; Liu, H.; Xiao, W.; Wang, Y.; Yuan, Y.
Engineering of beta-carotene hydroxylase and ketolase for astaxanthin overproduction in Saccharomyces cerevisiae (2017), Front. Chem. Sci. Engin., 11, 89-99 .

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Cloned(Commentary)

Cloned (Comment)	Organism
astaxanthin biosynthesis pathway is constructed in Saccharomyces cerevisiae by introducing heterologous CrtZ and CrtW into an existing high beta-carotene producing strain. Both genes crtZ and crtW are codon optimized and expressed under the control of constitutive promoters. Strain SyBE_Sc118060 with CrtW from Brevundimonas vesicularis DC263 and CrtZ from Alcaligenes sp. strain PC-1 achieves the highest astaxanthin yield of 3.1 mg/g dry cell weight	Alcaligenes sp. PC-1
expressed in Saccharomyces cerevisiae	Alcaligenes sp. PC-1

Cloned (Comment)

Organism

astaxanthin biosynthesis pathway is constructed in Saccharomyces cerevisiae by introducing heterologous CrtZ and CrtW into an existing high beta-carotene producing strain. Both genes crtZ and crtW are codon optimized and expressed under the control of constitutive promoters. Strain SyBE_Sc118060 with CrtW from Brevundimonas vesicularis DC263 and CrtZ from Alcaligenes sp. strain PC-1 achieves the highest astaxanthin yield of 3.1 mg/g dry cell weight

Alcaligenes sp. PC-1

expressed in Saccharomyces cerevisiae

Alcaligenes sp. PC-1

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2	Alcaligenes sp. PC-1	-	beta-cryptoxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2	Alcaligenes sp. PC-1	overall reaction	zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?
beta-cryptoxanthin + reduced ferredoxin [iron-sulfur] cluster + H+ + O2	Alcaligenes sp. PC-1	-	zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Alcaligenes sp. PC-1	-	-	-
Alcaligenes sp. PC-1	Q44262	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2	-	Alcaligenes sp. PC-1	beta-cryptoxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?
beta-carotene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2	overall reaction	Alcaligenes sp. PC-1	zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?
beta-cryptoxanthin + reduced ferredoxin [iron-sulfur] cluster + H+ + O2	-	Alcaligenes sp. PC-1	zeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?

Synonyms

Synonyms	Comment	Organism
beta-carotene hydroxylase	-	Alcaligenes sp. PC-1
CrtZ	-	Alcaligenes sp. PC-1