Literature summary for 1.14.15.24 extracted from

  • Hundle, B.; OBrien, D.; Beyer, P.; Kleining, H.; Hearst, J.
    In vitro expression and activity of lycopene cyclase and beta-carotene hydroxylase from Erwinia herbicola (1993), FEBS Lett., 315, 329-334.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pantoea agglomerans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm exclusively located in the cytoplasm Pantoea agglomerans
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
SDS-PAGE Pantoea agglomerans

Organism

Organism UniProt Comment Textmining
Pantoea agglomerans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
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Pantoea agglomerans zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
?
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
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Pantoea agglomerans beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?

Synonyms

Synonyms Comment Organism
beta-carotene hydroxylase
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Pantoea agglomerans

Cofactor

Cofactor Comment Organism Structure
additional information the beta-carotene hydroxylase reaction proceeds in the absence of externally added cofactors. However, electron donors, such as NAD, NADPH, and ascorbate were able to stimulate the reaction Pantoea agglomerans