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Literature summary for extracted from

  • Hundle, B.; OBrien, D.; Beyer, P.; Kleining, H.; Hearst, J.
    In vitro expression and activity of lycopene cyclase and beta-carotene hydroxylase from Erwinia herbicola (1993), FEBS Lett., 315, 329-334.
    View publication on PubMed


Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pantoea agglomerans


Localization Comment Organism GeneOntology No. Textmining
cytoplasm exclusively located in the cytoplasm Pantoea agglomerans 5737

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
SDS-PAGE Pantoea agglomerans


Organism UniProt Comment Textmining
Pantoea agglomerans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Pantoea agglomerans beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
Pantoea agglomerans zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O


Synonyms Comment Organism
beta-carotene hydroxylase
Pantoea agglomerans


Cofactor Comment Organism Structure
additional information the beta-carotene hydroxylase reaction proceeds in the absence of externally added cofactors. However, electron donors, such as NAD, NADPH, and ascorbate were able to stimulate the reaction Pantoea agglomerans