Literature summary for 1.14.15.24 extracted from

Fraser, P.D.; Shimada, H.; Misawa, N.
Enzymic confirmation of reactions involved in routes to astaxanthin formation, elucidated using a direct substrate in vitro assay (1998), Eur. J. Biochem., 252, 229-236.

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Activating Compound

Activating Compound	Comment	Organism	Structure
CHAPS	0.01% (v/v) promotes small enhancement in activity	Agrobacterium aurantiacum
deoxycholate	0.01% (v/v) promotes small enhancement in activity	Agrobacterium aurantiacum
NP-40	0.01% (v/v) promotes small enhancement in activity	Agrobacterium aurantiacum
sodium cholate	0.01% (v/v) promotes small enhancement in activity	Agrobacterium aurantiacum
Triton X-100	0.01% (v/v) promotes small enhancement in activity	Agrobacterium aurantiacum
Tween 60	0.01% (v/v) promotes small enhancement in activity	Agrobacterium aurantiacum

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Agrobacterium aurantiacum

Inhibitors

Inhibitors	Comment	Organism	Structure
CHAPS	1% (v/v) promotes inhibition of activity	Agrobacterium aurantiacum
deoxycholate	1% (v/v) promotes inhibition of activity	Agrobacterium aurantiacum
NP-40	1% (v/v) promotes inhibition of activity	Agrobacterium aurantiacum
SDS	SDS inhibits activity completely at 0.01% (v/v)	Agrobacterium aurantiacum
sodium cholate	1% (v/v) promotes inhibition of activity	Agrobacterium aurantiacum
Triton X-100	1% (v/v) promotes inhibition of activity	Agrobacterium aurantiacum
Tween 60	1% (v/v) promotes inhibition of activity	Agrobacterium aurantiacum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00056	-	echinenone	in 0.4 M Tris/HCl, pH 8.0, at 30°C	Agrobacterium aurantiacum
0.001	-	phoenicoxanthin	in 0.4 M Tris/HCl, pH 8.0, at 30°C	Agrobacterium aurantiacum
0.00182	-	3-hydroxyechinenone	in 0.4 M Tris/HCl, pH 8.0, at 30°C	Agrobacterium aurantiacum
0.0025	-	beta-carotene	in 0.4 M Tris/HCl, pH 8.0, at 30°C	Agrobacterium aurantiacum
0.004	-	3'-hydroxyechinenone	in 0.4 M Tris/HCl, pH 8.0, at 30°C	Agrobacterium aurantiacum

Organism

Organism	UniProt	Comment	Textmining
Agrobacterium aurantiacum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Agrobacterium aurantiacum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2	-	Agrobacterium aurantiacum	4-ketozeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O	4-ketozeaxanthin is adonixanthin	?
3-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2	-	Agrobacterium aurantiacum	4-ketozeaxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O	4-ketozeaxanthin is adonixanthin	?
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2	-	Agrobacterium aurantiacum	zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O	-	?
beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2	-	Agrobacterium aurantiacum	zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?
echinenone + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2	conversion occurs via 3- and 3'-hydroxyechinenone	Agrobacterium aurantiacum	4-ketozeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O	-	?
phoenicoxanthin + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2	phoenicoxanthin is adonirubin	Agrobacterium aurantiacum	astaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O	-	?

Synonyms

Synonyms	Comment	Organism
carotenoid (beta-ionone ring) 3,3'-hydroxylase	-	Agrobacterium aurantiacum
CrtZ	-	Agrobacterium aurantiacum

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Release 2023.1 (January 2023)