Literature summary for 1.14.15.24 extracted from

  • Bouvier, F.; Keller, Y.; DHarlingue, A.; Camara, B.
    Xanthophyll biosynthesis: Molecular and functional characterization of carotenoid hydroxylases from pepper fruits (Capsicum annuum L.) (1998), Biochim. Biophys. Acta, 1391, 320-328.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Capsicum annuum

Engineering

Protein Variants Comment Organism
H274A inactive Capsicum annuum
H184A inactive Capsicum annuum
H176A inactive Capsicum annuum
H171A inactive Capsicum annuum
H187A inactive Capsicum annuum
H188A inactive Capsicum annuum
H245A inactive Capsicum annuum
H250A inactive Capsicum annuum
H275A inactive Capsicum annuum

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline 5% residual activity at 1 mM Capsicum annuum
cytochrome c addition of cytochrome c to the incubation medium inhibits hydroxylase activity Capsicum annuum
o-phenanthroline 10% residual activity at 1 mM Capsicum annuum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000 26000 SDS-PAGE Capsicum annuum

Organism

Organism UniProt Comment Textmining
Capsicum annuum O49814
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, Talon nickel-affinity resin column chromatography, and Mono Q column chromatography Capsicum annuum

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Capsicum annuum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2 the hydroxylase activity is strictly dependent upon the presence of NADPH, ferredoxin, ferredoxin oxidoreductase, and catalase Capsicum annuum zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
?
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 the hydroxylase activity is strictly dependent upon the presence of NADPH, ferredoxin, ferredoxin oxidoreductase, and catalase Capsicum annuum beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?
beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 the hydroxylase activity is strictly dependent upon the presence of NADPH, ferredoxin, ferredoxin oxidoreductase, and catalase Capsicum annuum zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?

Synonyms

Synonyms Comment Organism
carotenoid hydroxylase 2
-
Capsicum annuum

Cofactor

Cofactor Comment Organism Structure
Ferredoxin the hydroxylase is strictly dependent upon reduced ferredoxin which cannot be replaced by NADPH Capsicum annuum