Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
SDS-PAGE | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phenyl Sepharose column chromatography, and hydroxyapatite column chromatography | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | upon in vitro reconstitution of CYP175A1 activity with purified recombinant flavodoxin and flavodoxin reductase from Escherichia coli, only very low beta-cryptoxanthin production is observed | Thermus thermophilus | beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | upon in vitro reconstitution of CYP175A1 activity with purified recombinant flavodoxin and flavodoxin reductase from Escherichia coli, only very low beta-cryptoxanthin production is observed | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2 | overall reaction, the enzyme catalyzes the hydroxylation of beta-carotene at 3- and 3'-positions | Thermus thermophilus | zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2 | overall reaction, the enzyme catalyzes the hydroxylation of beta-carotene at 3- and 3'-positions | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | - |
Thermus thermophilus | zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP175A1 | - |
Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
the enzyme activity disappears completely at 65°C | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Thermus thermophilus |