Literature summary for 1.14.15.24 extracted from

  • Momoi, K.; Hofmann, U.; Schmid, R.; Urlacher, V.
    Reconstitution of beta-carotene hydroxylase activity of thermostable CYP175A1 monooxygenase (2006), Biochem. Biophys. Res. Commun., 339, 331-336.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
SDS-PAGE Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl Sepharose column chromatography, and hydroxyapatite column chromatography Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2 overall reaction, the enzyme catalyzes the hydroxylation of beta-carotene at 3- and 3'-positions Thermus thermophilus zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
?
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2 overall reaction, the enzyme catalyzes the hydroxylation of beta-carotene at 3- and 3'-positions Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
?
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 upon in vitro reconstitution of CYP175A1 activity with purified recombinant flavodoxin and flavodoxin reductase from Escherichia coli, only very low beta-cryptoxanthin production is observed Thermus thermophilus beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2 upon in vitro reconstitution of CYP175A1 activity with purified recombinant flavodoxin and flavodoxin reductase from Escherichia coli, only very low beta-cryptoxanthin production is observed Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?
beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
Thermus thermophilus zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?
beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?

Synonyms

Synonyms Comment Organism
CYP175A1
-
Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
the enzyme activity disappears completely at 65°C Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
heme
-
Thermus thermophilus