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show all sequences of 1.14.15.22

Crystal structure of CYP105A1 (P450SU-1) in complex with 1alpha,25-dihydroxyvitamin D3

Sugimoto, H.; Shinkyo, R.; Hayashi, K.; Yoneda, S.; Yamada, M.; Kamakura, M.; Ikushiro, S.; Shiro, Y.; Sakaki, T.; Biochemistry 47, 4017-4027 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Streptomyces griseolus
Crystallization (Commentary)
Crystallization
Organism
wild-type to 1.5 A resolution, and mutant R84A, native protein and in complex with 1alpha,25-dihydroxyvitamin D3. The compound is positioned 11 A from the iron atom along the I helix within the pocket. The loss of two hydrogen bonds in the R84A mutant increases the adaptability of the B and F helices, creating a transient binding site
Streptomyces griseolus
Engineering
Amino acid exchange
Commentary
Organism
I243A
complete loss of activity
Streptomyces griseolus
I293A
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 5.4 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 3.4 mmol of product per min and mol of protein
Streptomyces griseolus
L180A
complete loss of activity
Streptomyces griseolus
R193A
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 0.48 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 0.72 mmol of product per min and mol of protein
Streptomyces griseolus
R193K
complete loss of activity
Streptomyces griseolus
R193Q
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 0.33 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 0.31 mmol of product per min and mol of protein
Streptomyces griseolus
R73A
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 30.9 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 20.8 mmol of product per min and mol of protein
Streptomyces griseolus
R84A
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 75.7 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 34.5 mmol of product per min and mol of protein
Streptomyces griseolus
R89A
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 0.25 mmol of product per min and mol of protein, no 25-hydroxyvitamin D3 hydroxylation activity at C1alpha
Streptomyces griseolus
S236A
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 4.6 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 3.4 mmol of product per min and mol of protein
Streptomyces griseolus
V181A
complete loss of activity
Streptomyces griseolus
V88A
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 1.52 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 0.34 mmol of product per min and mol of protein
Streptomyces griseolus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces griseolus
P18326
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
wild-type, 1alpha-hydroxyvitamin D3 hydroxylation activity at C25 2.8 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 2.1 mmol of product per min and mol of protein
Streptomyces griseolus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1alpha-hydroxyvitamin D3 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
737669
Streptomyces griseolus
1alpha,25-dihydroxyvitamin D3 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
25-hydroxyvitamin D3 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
737669
Streptomyces griseolus
1alpha,25-dihydroxyvitamin D3 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
vitamin D3 + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
737669
Streptomyces griseolus
1alpha-hydroxyvitamin D3 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
vitamin D3 + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
737669
Streptomyces griseolus
25-hydroxyvitamin D3 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Streptomyces griseolus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
wild-type to 1.5 A resolution, and mutant R84A, native protein and in complex with 1alpha,25-dihydroxyvitamin D3. The compound is positioned 11 A from the iron atom along the I helix within the pocket. The loss of two hydrogen bonds in the R84A mutant increases the adaptability of the B and F helices, creating a transient binding site
Streptomyces griseolus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
I243A
complete loss of activity
Streptomyces griseolus
I293A
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 5.4 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 3.4 mmol of product per min and mol of protein
Streptomyces griseolus
L180A
complete loss of activity
Streptomyces griseolus
R193A
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 0.48 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 0.72 mmol of product per min and mol of protein
Streptomyces griseolus
R193K
complete loss of activity
Streptomyces griseolus
R193Q
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 0.33 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 0.31 mmol of product per min and mol of protein
Streptomyces griseolus
R73A
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 30.9 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 20.8 mmol of product per min and mol of protein
Streptomyces griseolus
R84A
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 75.7 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 34.5 mmol of product per min and mol of protein
Streptomyces griseolus
R89A
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 0.25 mmol of product per min and mol of protein, no 25-hydroxyvitamin D3 hydroxylation activity at C1alpha
Streptomyces griseolus
S236A
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 4.6 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 3.4 mmol of product per min and mol of protein
Streptomyces griseolus
V181A
complete loss of activity
Streptomyces griseolus
V88A
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 1.52 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 0.34 mmol of product per min and mol of protein
Streptomyces griseolus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
wild-type, 1alpha-hydroxyvitamin D3 hydroxylation activity at C25 2.8 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 2.1 mmol of product per min and mol of protein
Streptomyces griseolus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1alpha-hydroxyvitamin D3 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
737669
Streptomyces griseolus
1alpha,25-dihydroxyvitamin D3 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
25-hydroxyvitamin D3 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
737669
Streptomyces griseolus
1alpha,25-dihydroxyvitamin D3 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
vitamin D3 + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
737669
Streptomyces griseolus
1alpha-hydroxyvitamin D3 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
vitamin D3 + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
737669
Streptomyces griseolus
25-hydroxyvitamin D3 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Other publictions for EC 1.14.15.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737618
Hayashi
Sequential hydroxylation of vi ...
Streptomyces griseolus
Biochem. Biophys. Res. Commun.
473
853-858
2016
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1
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2
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2
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5
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1
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5
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738203
Hayashi
Three-step hydroxylation of vi ...
Streptomyces griseolus
FEBS J.
277
3999-4009
2010
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1
1
1
1
-
-
4
-
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-
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4
-
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-
-
-
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4
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4
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1
1
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1
1
-
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-
-
4
-
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-
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-
-
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4
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4
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4
4
737668
Hayashi
Structure-based design of a hi ...
Streptomyces griseolus
Biochemistry
47
11964-11972
2008
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2
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2
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4
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2
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4
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737669
Sugimoto
Crystal structure of CYP105A1 ...
Streptomyces griseolus
Biochemistry
47
4017-4027
2008
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-
1
1
12
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4
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1
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4
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1
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1
12
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1
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4
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737586
Sawada
Conversion of vitamin D3 to 1a ...
Streptomyces griseolus
Biochem. Biophys. Res. Commun.
320
156-164
2004
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1
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3
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4
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1
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3
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1
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3
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1
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3
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