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Literature summary for 1.14.15.21 extracted from

  • Schaller, S.; Wilhelm, C.; Strzalka, K.; Goss, R.
    Investigating the interaction between the violaxanthin cycle enzyme zeaxanthin epoxidase and the thylakoid membrane (2012), J. Photochem. Photobiol. B Biol., 114, 119-125.
    View publication on PubMed
Search for more literature for 1.14.15.21

General Stability

General Stability Organism
there is a slight increase of the enzyme activity after the treatment with freeze-thaw cycles at pH 7.5, while thylakoids frozen and thawed a pH 5.2 show a slight reduction of the enzyme activity Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
NaBr there is a decrease of enzyme activity to 21% of the activity of control thylakoids when incubated once with 2 M NaBr. A second treatment with 2 M NaBr leads to an almost complete inhibition of enzyme activity Spinacia oleracea
octylglucoside complete inhibition at 30 mM Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
thylakoid membrane
-
 Spinacia oleracea 42651
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
antheraxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 Spinacia oleracea
-
 violaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
zeaxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 Spinacia oleracea
-
 antheraxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
antheraxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Spinacia oleracea violaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
zeaxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Spinacia oleracea antheraxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

Synonyms Comment Organism
ZEP
-
 Spinacia oleracea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 4 there is a slight increase of the enzyme activity after the treatment with freeze-thaw cycles at pH 7.5, while thylakoids frozen and thawed a pH 5.2 show a slight reduction of the enzyme activity Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Spinacia oleracea
NADH
-
 Spinacia oleracea