BRENDA - Enzyme Database show
show all sequences of 1.14.15.21

FAD is a further essential cofactor of the NAD(P)H and O2-dependent zeaxanthin-epoxidase

Büch, K.; Stransky, H.; Hager, A.; FEBS Lett. 376, 45-48 (1995)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
diphenylene iodonium chloride
IC50: 0.0023 mM
Spinacia oleracea
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
thylakoid
stromal side
Spinacia oleracea
9579
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Spinacia oleracea
-
L. cv. Butterfly
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Spinacia oleracea
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
antheraxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
658690
Spinacia oleracea
violaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
zeaxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
658690
Spinacia oleracea
antheraxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
essential cofactor
Spinacia oleracea
NAD(P)H
-
Spinacia oleracea
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0023
-
IC50: 0.0023 mM
Spinacia oleracea
diphenylene iodonium chloride
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
essential cofactor
Spinacia oleracea
NAD(P)H
-
Spinacia oleracea
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0023
-
IC50: 0.0023 mM
Spinacia oleracea
diphenylene iodonium chloride
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
diphenylene iodonium chloride
IC50: 0.0023 mM
Spinacia oleracea
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
thylakoid
stromal side
Spinacia oleracea
9579
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Spinacia oleracea
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
antheraxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
658690
Spinacia oleracea
violaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
zeaxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
658690
Spinacia oleracea
antheraxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Other publictions for EC 1.14.15.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738920
Eilers
Identification of genes coding ...
Phaeodactylum tricornutum, Phaeodactylum tricornutum CCAP 1055/1
J. Plant Physiol.
192
64-70
2016
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-
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8
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1
2
-
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-
739289
Zhang
MsZEP, a novel zeaxanthin epox ...
Medicago sativa
Plant Cell Rep.
35
439-453
2016
-
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1
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1
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1
2
-
-
739361
Gonzalez-Jorge
ZEAXANTHIN EPOXIDASE activity ...
Arabidopsis thaliana
Plant Physiol.
171
1837-1851
2016
-
-
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1
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1
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739281
Schwarz
Tissue-specific accumulation a ...
Arabidopsis thaliana
Plant Cell Physiol.
56
346-357
2015
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-
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3
-
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3
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3
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739124
Zhao
Molecular characterization of ...
Lycium chinense
Molecules
19
11250-11262
2014
-
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728184
Schaller
Investigating the interaction ...
Spinacia oleracea
J. Photochem. Photobiol. B Biol.
114
119-125
2012
-
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1
2
-
1
-
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2
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2
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1
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-
699796
Nowicka
New transgenic line of Arabido ...
Arabidopsis thaliana
J. Plant Physiol.
166
1045-1056
2009
-
-
-
-
1
-
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-
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2
-
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2
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-
1
1
-
-
-
700645
Esteban
Distribution and evolutionary ...
Arabidopsis thaliana, Citrus unshiu, Solanum lycopersicum, Solanum tuberosum
Physiol. Plant.
135
379-389
2009
-
-
4
-
-
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-
-
-
-
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-
10
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-
4
4
7
7
-
-
700831
Vallabhaneni
Timing and biosynthetic potent ...
Zea mays
Plant Physiol.
150
562-572
2009
-
-
-
-
-
-
-
-
-
-
-
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7
-
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5
-
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2
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-
-
1
-
-
1
-
-
684131
Kruk
Occurrence of neoxanthin and l ...
Cuscuta campestris, Cuscuta epithymum, Cuscuta europaea, Cuscuta gronovii, Cuscuta japonica, Cuscuta lupuliformis, Cuscuta platyloba, Cuscuta reflexa
Acta Biochim. Pol.
55
183-190
2008
-
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-
24
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12
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8
8
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32
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24
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24
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24
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8
8
-
32
-
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-
684935
Park
Overexpression of Arabidopsis ...
Arabidopsis thaliana
Biochem. Biophys. Res. Commun.
375
80-85
2008
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1
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689406
Wang
Overexpression of zeaxanthin e ...
Solanum lycopersicum
Physiol. Plant.
132
384-396
2008
1
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1
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1
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1
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2
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1
1
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4
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6
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6
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1
1
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689544
Galpaz
Abscisic acid deficiency in th ...
Solanum lycopersicum
Plant J.
53
717-730
2008
-
-
-
-
1
-
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1
-
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3
-
3
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2
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2
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3
-
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689601
Toh
High temperature-induced absci ...
Arabidopsis thaliana
Plant Physiol.
146
1368-1385
2008
1
-
1
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2
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1
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1
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1
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3
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-
699257
Clotault
Expression of carotenoid biosy ...
Daucus carota
J. Exp. Bot.
59
3563-3573
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
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1
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1
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700886
Coesel
Evolutionary origins and funct ...
Phaeodactylum tricornutum
PLoS ONE
3
e2896
2008
-
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1
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10
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6
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6
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1
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3
-
-
675658
Latowski
Amino sugars - new inhibitors ...
Lemna trisulca
J. Plant Physiol.
164
231-237
2007
-
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1
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1
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1
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676549
Destefano-Beltran
Effects of postharvest storage ...
Solanum tuberosum
Plant Mol. Biol.
61
687-697
2006
-
-
1
-
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1
3
-
3
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2
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3
1
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1
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1
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1
1
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3
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2
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3
1
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1
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676715
Frey
Regulation of carotenoid and A ...
Nicotiana plumbaginifolia
Planta
224
622-632
2006
-
-
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1
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3
-
3
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1
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1
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3
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675123
Barrero
A mutational analysis of the A ...
Arabidopsis thaliana
J. Exp. Bot.
56
2071-2083
2005
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1
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1
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3
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3
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1
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3
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1
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1
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1
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1
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3
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675250
Latowski
Inhibition of zeaxanthin epoxi ...
Lemna trisulca, Prunus armeniaca, Solanum lycopersicum
J. Inorg. Biochem.
99
2081-2087
2005
-
-
-
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3
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3
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6
-
8
-
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2
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8
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3
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3
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3
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3
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3
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3
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6
-
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2
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8
-
3
-
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3
-
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676583
Charron
Identification, expression, an ...
Debaryomyces hansenii, Pyropia yezoensis, Triticum aestivum
Plant Physiol.
139
2017-2028
2005
1
-
1
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2
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3
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5
-
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1
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3
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1
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3
1
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3
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3
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3
-
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3
-
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660302
Matsubara
Occurrence of the lutein-epoxi ...
Amyema cambagei, Amyema miquelii, Amyema pendulum, Arceuthobium divaricatum, Arceuthobium oxycedri, Cassytha filiformis, Exocarpos cupressiformis, Melampyrum pratense, Muellerina eucalyptoides, Nuytsia floribunda, Phoradendron californicum, Phoradendron dipterum, Phoradendron emarginatum, Phoradendron hexastichon, Phoradendron juniperinum, Phoradendron perrottetii, Phoradendron piauhyanum, Phoradendron piperoides, Phoradendron semivenosum, Phoradendron tunaeforme, Phoradendron undulatum, Phthirusa ovata, Psittacanthus dichrous, Psittacanthus robustus, Struthanthus flexicaulis, Struthanthus marginatus, Viscum album, Viscum laxum
Planta
217
868-879
2003
-
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56
-
32
-
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56
-
-
-
-
-
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-
-
28
-
-
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28
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56
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56
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660295
Frommolt
The de-epoxidase and epoxidase ...
Mantoniella squamata
Planta
213
446-456
2001
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2
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2
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658155
Hieber
Plant lipocalins: violaxanthin ...
Arabidopsis sp., Capsicum annuum, Prunus americana, Solanum lycopersicum, Solanum lycopersicum Mill.
Biochim. Biophys. Acta
1482
84-91
2000
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13
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10
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4
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4
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10
-
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660190
Thompson
Abscisic acid biosynthesis in ...
Solanum lycopersicum
Plant Mol. Biol.
42
833-845
2000
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4
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2
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1
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659134
Bugos
Xanthophyll cycle enzymes are ...
Arabidopsis thaliana
J. Biol. Chem.
273
15321-15324
1998
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2
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3
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2
1
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659598
Burbidge
-
Structure and expression of a ...
Solanum lycopersicum, Solanum lycopersicum Mill.
J. Exp. Bot.
48
1749-1750
1997
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1
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4
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3
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11
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1
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1
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4
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11
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658536
Marin
Molecular identification of ze ...
Nicotiana plumbaginifolia
EMBO J.
15
2331-2342
1996
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1
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6
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2
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658690
Büch
FAD is a further essential cof ...
Spinacia oleracea
FEBS Lett.
376
45-48
1995
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1
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1
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1
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1
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658152
Gruszecki
-
LHCII, the major light-harvest ...
Secale cereale
Biochim. Biophys. Acta
1144
97-101
1993
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1
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