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Literature summary for 1.14.15.20 extracted from

  • Cornejo, J.; Willows, R.; Beale, S.
    Phytobilin biosynthesis: Cloning and expression of a gene encoding soluble ferredoxin-dependent heme oxygenase from Synechocystis sp. PCC 6803 (1998), Plant J., 15, 99-107.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Synechocystis sp. PCC 6803

Inhibitors

Inhibitors Comment Organism Structure
Sn-protoporphyrin IX 25% inhibition at 0.02 mM Synechocystis sp. PCC 6803

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
-
Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
mesoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Synechocystis sp. PCC 6803
-
mesobiliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?

Organism

Organism UniProt Comment Textmining
Synechocystis sp. PCC 6803
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification by nickel chelating column chromatography Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mesoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
Synechocystis sp. PCC 6803 mesobiliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
?

Subunits

Subunits Comment Organism
? x * 28540, isoform HO2, calculated from amino acid sequence Synechocystis sp. PCC 6803
? x * 31000, isoform HO2, SDS-PAGE Synechocystis sp. PCC 6803

Synonyms

Synonyms Comment Organism
ferredoxin-dependent heme oxygenase
-
Synechocystis sp. PCC 6803
Ho1 isoform Synechocystis sp. PCC 6803
Ho2 isoform Synechocystis sp. PCC 6803

Expression

Organism Comment Expression
Synechocystis sp. PCC 6803 enzyme expression increases about 7fold during incubation of cells for 72 h in iron-deficient medium up